Second: crossing overt the USP method to our sample testing:
1- Linearity: we need to have a linearity curve for that method, the curve must include the standard concentration equivalent to our sample concentration. The curve also should include a total of five levels (50%, 75%, 100%, 125%, and 150% of the target concentration “based on the sample preparations”)
2- Specificity: injecting sample , standard , standard spiked with 25% sample. Examine that there