The purpose of this experiment was to make copies of DNA. Each student has tube containing Taq polymerase, dNTP’s, polymerase buffer, 27F primer, 1492R primer and di water. We mixed isolated bacteria by using a toothpick into 5 μL di of sterile water. Add 1 μL of the mixed colony with DI water into the tube. While performing this experiment we did a gram stain of the same colony. The last thing we did was gel electrophoresis. The purpose of this experiment was to separate DNA, according to its size. For this part of an experiment, we mixed 5 μL of sample with 1 μL of iodine so that the DN A will not float. Instead it will stay inside the well. Then transfer 5 μL of the mixture to designated well. Turn on the gel electrophoresis for 10 mins until it finishes running. After it finishes running send the results to sequencing company. At last take the result from sequencing company and Nucleotide BLAST it to get the genus and the species of the
The purpose of this experiment was to make copies of DNA. Each student has tube containing Taq polymerase, dNTP’s, polymerase buffer, 27F primer, 1492R primer and di water. We mixed isolated bacteria by using a toothpick into 5 μL di of sterile water. Add 1 μL of the mixed colony with DI water into the tube. While performing this experiment we did a gram stain of the same colony. The last thing we did was gel electrophoresis. The purpose of this experiment was to separate DNA, according to its size. For this part of an experiment, we mixed 5 μL of sample with 1 μL of iodine so that the DN A will not float. Instead it will stay inside the well. Then transfer 5 μL of the mixture to designated well. Turn on the gel electrophoresis for 10 mins until it finishes running. After it finishes running send the results to sequencing company. At last take the result from sequencing company and Nucleotide BLAST it to get the genus and the species of the