Table 1. Changes in the relative intensities of peptides with compound 1 at various concentrations …show more content…
In this approach, the molar fraction of KPNB1 and compound 2 was varied, while keeping the total concentration ([KPNB1]+[2]) constant. The fluorescence intensity was measured at 520 nm, and the changes in the fluorescence intensity were plotted versus the molar fraction of KPNB1 in PBS (pH 8.0), showing that linear regression fittings were made at the two extremes of the curve (Figure 2). The maximum fluorescence change were observed at the 1/2 molar fraction of 2, suggesting that compound 2 and KPNB1 formed the complex with a 1:1