Phosphorylation of glucose to glucose-6-phosphate uses one molecule of ATP.
Phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate uses on molecule of ATP.
Because the process will only stop at the glycolytic pathway, the following amount of ATPs will be produced at the energy payoff phase of glycolysis:
2 molecules of NADH are produced in the reaction involving oxidation of glyceraldehyde phosphate to 1,3-bisphosphoglycerate. The 2 molecules of NADH will enter the electron transfer chain reaction to produce 6 ATP.
2 molecules of ATP is produced in the substrate level phosphorylation reaction in which 1,3-bisphosphoglycerate to 3-phosphoglycerate.
2 molecules