In the test tube #1, there was a few bubbles compared to the tube #2. The difference in the reactions was in test tube #1, the catalase was denatured by the high temperature, but in test tube #2, there was no denatured occurred, so the catalase could convert hydrogen peroxide into water and oxygen. The optimal condition for catalase in this experiment is the temperature should be not too high, so the catalase can’t be denatured. In the experiment 4.4, we could see the effect of pH on enzyme activity by adding 2ml hydrogen peroxide and a small piece of liver to each test tube and adding 20 drops of acidic solution into tube #1, adding 20 drops of dH2O (pH 7) into tube #2, and adding 20 drops of basic solution into tube #3. We observed that the test tube #2 had the greatest bubbles, and the second one was the test tube #1, the third one was the test tube #3. We concluded that because pH 7 solution is neutral, it means no extra H+ or – OH ions to block the active site on catalase like in the test tube #1 and #3, they were lack of reaction due to extra H+ or -OH in pH4 and Ph11 solution blocking the active site, so the substrate can’t bind. The optimal condition for catalase in this experiment is enzymes are very sensitive to pH because enzymes have an optimal range at which
In the test tube #1, there was a few bubbles compared to the tube #2. The difference in the reactions was in test tube #1, the catalase was denatured by the high temperature, but in test tube #2, there was no denatured occurred, so the catalase could convert hydrogen peroxide into water and oxygen. The optimal condition for catalase in this experiment is the temperature should be not too high, so the catalase can’t be denatured. In the experiment 4.4, we could see the effect of pH on enzyme activity by adding 2ml hydrogen peroxide and a small piece of liver to each test tube and adding 20 drops of acidic solution into tube #1, adding 20 drops of dH2O (pH 7) into tube #2, and adding 20 drops of basic solution into tube #3. We observed that the test tube #2 had the greatest bubbles, and the second one was the test tube #1, the third one was the test tube #3. We concluded that because pH 7 solution is neutral, it means no extra H+ or – OH ions to block the active site on catalase like in the test tube #1 and #3, they were lack of reaction due to extra H+ or -OH in pH4 and Ph11 solution blocking the active site, so the substrate can’t bind. The optimal condition for catalase in this experiment is enzymes are very sensitive to pH because enzymes have an optimal range at which