A clear orange layer of Lycopene band formed at the beginning of the separation; however there were not enough pigments for collection. The band became more blurred when it reached the bottom. This was due to small amount of pigment obtained during filtration after drying.
Iodine was added after examining the Lycopene sample using UV spectrum which was to Isomerise the Lycopene sample to mixture of both cis and trans Isomers. This solution was then …show more content…
The reagent phenylmagnesium bromide is then added with benzophenone and a magnesium salt precipitate forms. Finally the salt is converted into triphenylmethanol by adding water, and acid is added to dissolve any basic product. Grignard Reagents are also used in the following important reactions: Grignard reagent can be added in excess to an ester or lactone to give a tertiary alcohol in which two alkyl groups are the same, and the addition of a Grignard reagent to a nitrile produces an unsymmetrical ketone via a metalloimine