There were 15,732 participants in the study. Four US communities The Field centers were randomly selected and a sample of approximately 4,000 individuals aged 45-64 years were recruited in Forsyth County, North Carolina, Jackson, Mississippi , Minneapolis suburbs, Minnesota , and Washington county, Maryland. First, participants were examined to yield medical and socio-demographic data and follow ups were on-going, every 3 years. The participants would fast 12 hours before their examination visits. The lipid testing was done by the Central Lipid Laboratory in Houston, Texas. When it comes to total cholesterol, that was determined by enzymatic methods with high density lipoprotein cholesterol measured after dextran-magnesium precipitation. Systolic blood pressure was measured three times, five minutes apart using a random zero sphygmomanometer while participants were seated, and the average of the measures was used for the analysis. Information on each participant smoking status and antihypertensive medication use were obtained by self-report. Data regarding coronary heart disease events and risk factors were collected through annual telephone interviews, surveys of hospital discharge data, and death certificates from state vital statistics
There were 15,732 participants in the study. Four US communities The Field centers were randomly selected and a sample of approximately 4,000 individuals aged 45-64 years were recruited in Forsyth County, North Carolina, Jackson, Mississippi , Minneapolis suburbs, Minnesota , and Washington county, Maryland. First, participants were examined to yield medical and socio-demographic data and follow ups were on-going, every 3 years. The participants would fast 12 hours before their examination visits. The lipid testing was done by the Central Lipid Laboratory in Houston, Texas. When it comes to total cholesterol, that was determined by enzymatic methods with high density lipoprotein cholesterol measured after dextran-magnesium precipitation. Systolic blood pressure was measured three times, five minutes apart using a random zero sphygmomanometer while participants were seated, and the average of the measures was used for the analysis. Information on each participant smoking status and antihypertensive medication use were obtained by self-report. Data regarding coronary heart disease events and risk factors were collected through annual telephone interviews, surveys of hospital discharge data, and death certificates from state vital statistics