For experiment 6 the solution from the previous experiment is needed, varying tubes, master mix, ice, and a PCR machine. For experiment 7 varying tubes are needed, the product from the previous lab, pipette, ice, heat block, SOC, and incubator. For experiment 8 a agarose gel is needed along with ice, pipette, toothpick, centrifuge, vortex, buffer P1, buffer P2, buffer N3, buffer PE, and sterile water. For experiment 9 a NanoDrop machine is needed, a pipette, buffer, and incubator. Lastly for experiment 10 the plasmid DNA from the previous experiment is required along with a pipette; a primer provided by the TA, and a master mix (Garey et al.,
For experiment 6 the solution from the previous experiment is needed, varying tubes, master mix, ice, and a PCR machine. For experiment 7 varying tubes are needed, the product from the previous lab, pipette, ice, heat block, SOC, and incubator. For experiment 8 a agarose gel is needed along with ice, pipette, toothpick, centrifuge, vortex, buffer P1, buffer P2, buffer N3, buffer PE, and sterile water. For experiment 9 a NanoDrop machine is needed, a pipette, buffer, and incubator. Lastly for experiment 10 the plasmid DNA from the previous experiment is required along with a pipette; a primer provided by the TA, and a master mix (Garey et al.,