Step 2. Observe adults First, to observe the adults, two capped glass vials containing culture medium and Drosophila from larva to adult stages were obtained. Each of the two vials was carefully observed one at a time for natural and mating behavior.…
. Why does (or doesn’t) the frequency of a physical trait change in a rabbit population in different environments? c. This is an important investigation as understanding how populations are affected by different traits helps to understand why certain species thrive in an a certain an environment and why others don’t. This is an interesting investigation as there are no predators in this investigation, the rabbits are competing for food and that is why they are dying at an alarming rate.…
In the four-week long experiment conducted in the Bio 13 Lab, we were able to conduct a genetic analysis of the yeast S. cerevisiae, particularly investigating the inheritance and linkage of three genes: HIS4, LEU2, and LYS2. In week one, a suspension of spores was received and aliquots of the suspension were plates onto two YPD plates. After a week of yeast growth, haploid spore clones were carefully picked from the plates by sterile toothpicks and used to make patches on a new YPD plate, the master plate. In week three, the master plate was replicated onto three dropout plates: -His, -Leu, and -Lys. In the final week, the growth of the patches on the dropout plates were analyzed and class data was collected in order to conduct calculations for…
The posterior scutellar bristles in D. melanogaster are two long bristles attached to the end of the scutellum and projected in an outward V-shape. Yet, in hot cheeto, the V-shape projection is not present because the short bristles curled to opposite sides of the scutellum. Humeral bristles, dorsocentral bristles, notopleural bristles, anterior scutellar bristles, orbital bristles, and ocellar bristles are all affected by this mutation. This mutation affects the phenotype equally in both female and male mutants. However, the mortality rate in hot cheeto females was greater than males.…
F f F FF Ff f Ff ff 75% of wild type is Dominant and 25% is recessive. Activity 3 – F2 Generation Observations Summarize the procedures that were conducted during this final week of this…
The effects of gene mutation on Olfactory Sensory Neurons in Caenorhabditis Elegans Amira Brown University of California, Santa Barbara Abstract This experiment examines the effects of distinct odorants on chemotaxis behavior of the nematode worm, Caenorhabditis elegans (C. Elegans). Nematode worms are appropriate for sensory physiology studies because its neuronal wiring is similar to that of humans. We focused on the role of three sensory neurons in the nose of the nematode—the 1) AWA and 2)…
Materials. Numerous substances in the experiment were used. The most frequently used was the unknown due to the need to test its physical and chemical qualities. When a solution of the unknown was made, 1.000 g of the unknown and 1.0 mL of water was used to make it. To test for the possible ions, 1.0 mL of silver nitrate and 1.0 mL of nitric acid were used for the ion test.…
As a group we have taken two sterile plastic vials and placed a cup of Drosophila medium powder into each one. After adding a cup of the medium with added half a cup of distillated water into the plastic vial, which allowed the medium to dissolve into it. We allowed a few minutes for the media to be absorbed and afterwards we applied a cotton plug to close the entrance to the vial. Anesthetizing the Drosophila flies In the lab, we had the fruit flies already asleep in a green bucket filled all the way up with ice.…
Melittin is the active component of bee venom and when injected releases histamine from mast cells and ruptures red blood cell corpuscles. This interaction is responsible for the initial pain and swelling that is associated with a bee sting. (Collison, 41) However, the properties that make melittin toxic can also be harnessed for therapeutic purposes. Melittin is a small protein containing 26 amino acids that comprises 50% of bee venom’s dry weight.…
Determining the affects of natural selection on gall fly (Eurosta) larvae from different predators Ariel O’Neil Biology 1020H Abstract This lab was done with the objective of determining if directional selection causes early larvae death in the gall fly. By first examining the parasitoid wasp and birds predatory actions we can hypothesize and predict the smaller galls will be most likely to survive directional selection.…
We hypothesized that curly wing flies would have difficulty courting past the flapping of its wings to create a “love song”. The FraxE flies are model organisms of a mutation that causes mental retardation and various behavioral problems. We did not expect very much courtship at all in this group. Lilli flies were similar to the FraxE model except they had the protein that was lost with the mutation placed roughly 50% back into them. We thought the flies with this mutation would have more courtship then the FraxE but less than the curly wing fly.…
The purpose of this lab is identify a particular strain of a mutation in C. elegans and determine its mode of inheritance. This is done by conducting a series of crosses between worms possessing the mutation and analyzing the phenotypes and genotypes. Genotypes are essentially the genetic code of an organism, while phenotypes are the physical traits an organism displays. Which phenotypes are expressed and how they are expressed is determined by genotypes. C. (Caenorhabditis) elegans are tiny, non-parasitic, transparent roundworms that primarily feed on bacteria such as E. coli.…
An unknown sample #18 was obtained on November 6, 2017. The sample came in a glass test tube with a cotton swab that contain the unknown sample. To find the identity of the unknown sample, we had to conduct several biochemical tests to eliminate and narrow down possible organisms that fit the biochemical description to accurately identify our unknown organism of interest. These biochemical tests provided insight on the physical color retention of our unknown sample, and through’s the unknown organism’s ability to break down tested compounds to determine if it had the corresponding enzyme that would allow it to do so. The testing of unknown organisms to accurately discover its identity is crucial in the field of health science.…
For this lab we conducted a population genetics study on the frequency of wild and apterous flies within a small population of fruit flies. The fruit fly Drosophila works well for genetic and evolutionary geared studies because they have a relatively short generation time but are intricate enough to reveal some biological principles that are parallel to many different kinds of eukaryotic organisms. When exposed to artificial selection in laboratory experiments, fruit flies have experienced dramatic changes behavioral and evolutionary traits within ten generations or less (Goldsmith, 1991). It is important to note that wild flies have wings and apterous flies do not. The purpose of artificial selection is for humans to modify species over generations by intentionally breeding two organisms with the objective of acquiring a specific trait.…
The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if it’s positive or negative. Performing aseptic techniques is the most crucial step that requires the utilizing of transferring, inoculating, and storing bacterial cultures and media. Aseptic technique is defined as procedures that prevent contamination…