The molecular ruler, as seen in Figure 1, did not produce bands that were as bold as the bands produce by the PCR products. This most likely occurred due to the sample not being loaded correctly into the sample well. Some of the contents may have diffused out of the well before the electrophoresis was started, causing lighter bands. Also, there may have been a pipetting error. Loading a smaller amount of the ruler could have caused these results. This study also reveals the many practical applications of PCR. PCR effectively amplifies a target DNA sequence to produce millions of copies. This can be used to produce plasmids for genetic transformations. The target gene can be cloned through PCR and the appropriate primers. Then gel electrophoresis can be used to isolate or separate the DNA fragments, after which the fragments can be inserted into plasmids for genetic transformations. PCR can also be used to diagnose patients with genetic disorders. This can be done by amplifying the problem DNA sequence to test for its presence in the DNA of a
The molecular ruler, as seen in Figure 1, did not produce bands that were as bold as the bands produce by the PCR products. This most likely occurred due to the sample not being loaded correctly into the sample well. Some of the contents may have diffused out of the well before the electrophoresis was started, causing lighter bands. Also, there may have been a pipetting error. Loading a smaller amount of the ruler could have caused these results. This study also reveals the many practical applications of PCR. PCR effectively amplifies a target DNA sequence to produce millions of copies. This can be used to produce plasmids for genetic transformations. The target gene can be cloned through PCR and the appropriate primers. Then gel electrophoresis can be used to isolate or separate the DNA fragments, after which the fragments can be inserted into plasmids for genetic transformations. PCR can also be used to diagnose patients with genetic disorders. This can be done by amplifying the problem DNA sequence to test for its presence in the DNA of a