Overall, there would be four Western blots completed. There would be two experimental Western blots completed: blot one would contain the ER fraction and blot two would contain the transmembrane fraction. Both blot one and two would be exposed to primary and secondary antibodies (one primary Ab would be specific for MH1 and one primary Ab would be specific for MH2). There would be two control Western blots completed: blot three would contain the ER fraction and blot four would contain the transmembrane fraction. Both blot three and four would not be exposed to primary or secondary antibodies (blocks would be used to supplement the
Overall, there would be four Western blots completed. There would be two experimental Western blots completed: blot one would contain the ER fraction and blot two would contain the transmembrane fraction. Both blot one and two would be exposed to primary and secondary antibodies (one primary Ab would be specific for MH1 and one primary Ab would be specific for MH2). There would be two control Western blots completed: blot three would contain the ER fraction and blot four would contain the transmembrane fraction. Both blot three and four would not be exposed to primary or secondary antibodies (blocks would be used to supplement the