PALCAM medium was formulated by Van Netten et al.in 1989 to overcome the insufficient selectivity of media used in the past for the detection and enumeration of Listeria. Peptones and yeast extract favor the excellent growth of Listeria. Glucose and starch are the energy sources for microbial development. Sodium chloride maintains osmotic balance. Listeria hydrolyze esculin to glucose and esculetin, the latter compound forming a black complex with ferric ions supplied by ferric citrate (Biokar Diagnostics,
PALCAM medium was formulated by Van Netten et al.in 1989 to overcome the insufficient selectivity of media used in the past for the detection and enumeration of Listeria. Peptones and yeast extract favor the excellent growth of Listeria. Glucose and starch are the energy sources for microbial development. Sodium chloride maintains osmotic balance. Listeria hydrolyze esculin to glucose and esculetin, the latter compound forming a black complex with ferric ions supplied by ferric citrate (Biokar Diagnostics,