Just like the previous experiments, all steps of aseptic technique were followed. The inoculated tubes were placed in the incubator for a 24 -hour period to determine how each organism reacted to the different degrees of pH. This is determined by the Spectrophotometer measuring the optical density of each culture. The last experiment of this lab tested the effect different osmotic pressures have on organisms. Materials used to perform this experiment included:
Gloves
Culture Medium
2 NaCl Agar plates
Cotton tip applicators
Bio-hazard disposal
Incubator shelf