Opuntia ficus-indica (L.) Mill belongs to the family Cactaceae. Its globally common name is the sweet prickly pear locally is called Albarshom. It has widely international spread. Firstly, Opuntia ficus-indica has founded Central America (Mexico). And later the species have been introduced to many areas of savanna regions and inhibit South Africa, Asia, and Australia as an ornamental, for camel feed (Brutsch and Zimmermann, 1993) and for its delicious fruits as food for humans, Vervet monkeys and baboons. O. ficus is succulent shrub or tree reaches to 1.5- 3 meters high which may be reached to 5 meters, propagated by seeds or seedlings. Woody trunks become stiff with age, have flat branches called Cladodes afford spines or may …show more content…
This may include some mandatory control measures. The study also aims at the effect of different heights on the genetic diversity of plants O. ficus and the study of biodiversity within this species. Where it is important in the ecosystem of the reserve. Using a technique inter-simple sequence repeat (ISSR) (Spooner,2005). This technique applied genetic identity study of O. ficus and useful for taxonomic studies of closely related O. ficus species. In addition, ISSRs involving gene mapping studied of the O. ficus. ISSRs are DNA fragments located inversely oriented. DNA segments of about 100-3000 bp amplified by PCR and flanked by closely spaced microsatellite sequences (DNA repeated sequence are short in specific locus) by a single primer or a pair of primers. ISSR is fast, effective and highly informational technique. They are considered reliable for DNA fingerprinting and by using Random Amplified Polymorphic DNA (RAPD) technique can be recognized to detect any genetic changes at the individual level as well as to clarify any close relationship between O. ficus species. To consider also their importance to fill gaps of gene …show more content…
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