when we extracted DNA, we used Proteinase K (Pro K) digests to mix it with it and touch spinning it to extract all genomic DNA. We added to it Lysis before just to make sure there is no damage to the DNA membrane. We amplified DNA sequences of our cheek cells using polymerase chain reaction technique. We placed our DNA that contains master mix on the ice container so it does not get denatured while setting up out the PCR mixture. As a group, we set up our master mix and all all the mixtures we need in it to decrease the percentage of errors which could be done. While making the mixture for master mix, we had to keep it on ice all the time. We had to do it twice because the first time was not successful, we had too much bubbles. The second time, we had a little bit of bubbles, but we tried to get rid of it by pipetting it out of the tube which was a big error. Doing that, made us not have enough master mix later on to add it to our extracted DNA and the negative control tube so when we looked at out gel under the light, we had no bands for negative
when we extracted DNA, we used Proteinase K (Pro K) digests to mix it with it and touch spinning it to extract all genomic DNA. We added to it Lysis before just to make sure there is no damage to the DNA membrane. We amplified DNA sequences of our cheek cells using polymerase chain reaction technique. We placed our DNA that contains master mix on the ice container so it does not get denatured while setting up out the PCR mixture. As a group, we set up our master mix and all all the mixtures we need in it to decrease the percentage of errors which could be done. While making the mixture for master mix, we had to keep it on ice all the time. We had to do it twice because the first time was not successful, we had too much bubbles. The second time, we had a little bit of bubbles, but we tried to get rid of it by pipetting it out of the tube which was a big error. Doing that, made us not have enough master mix later on to add it to our extracted DNA and the negative control tube so when we looked at out gel under the light, we had no bands for negative