Transformation allows the bacteria to be introduced to a foreign plasmid. The bacteria is then amplified by the plasmid, allowing for larger quantities of it to exist. Plasmids are small circular pieces of DNA that contain genetic information that may enhance the growth of bacteria. The information is a protein, that when encoded, will make the bacteria, in this E. coli, resistant to any type of antibiotic. Plasmids came about as a result of bacteria evolving. Bacteria is most often grown in the same type of environment that contains molds and fungi, leading them to all compete for the same nutrients. Mold and fungi have evolved to make toxins in order to kill the bacteria off, leaving them with more food. Bacteria, in turn, then evolved to making proteins that shuts down the toxins, still making them a healthy competitor for food in the environment. A plasmid containing resistance to any antibiotic, it 's usually ampicillin, is called a vector. The gene of interest is placed into the vector plasmid, which is then placed into the, already sensitive to ampicillin, E. coli. The bacteria is then spread over an agar plate that contains ampicillin. The ampicillin then applies pressure to the bacteria that has acquired resistance to ampicillin, causing it to grow and continually replicate. There is colony growth, which is when there are individual spots of bacterial
Transformation allows the bacteria to be introduced to a foreign plasmid. The bacteria is then amplified by the plasmid, allowing for larger quantities of it to exist. Plasmids are small circular pieces of DNA that contain genetic information that may enhance the growth of bacteria. The information is a protein, that when encoded, will make the bacteria, in this E. coli, resistant to any type of antibiotic. Plasmids came about as a result of bacteria evolving. Bacteria is most often grown in the same type of environment that contains molds and fungi, leading them to all compete for the same nutrients. Mold and fungi have evolved to make toxins in order to kill the bacteria off, leaving them with more food. Bacteria, in turn, then evolved to making proteins that shuts down the toxins, still making them a healthy competitor for food in the environment. A plasmid containing resistance to any antibiotic, it 's usually ampicillin, is called a vector. The gene of interest is placed into the vector plasmid, which is then placed into the, already sensitive to ampicillin, E. coli. The bacteria is then spread over an agar plate that contains ampicillin. The ampicillin then applies pressure to the bacteria that has acquired resistance to ampicillin, causing it to grow and continually replicate. There is colony growth, which is when there are individual spots of bacterial