Bacteria are the best suited organisms for the transformation process because they are single-cell organisms and are able of reproducing quickly, creating a fast production of daughter cells containing the desired genes. Fast production of offspring allows quickly assess if the new trait has been passed on. Bacteria naturally contain one or more small circular pieces of DNA called plasmids. Plasmid DNA usually contains genes for one or more traits that may be beneficial to bacterial survival. In nature, bacteria can transfer plasmids back and forth, allowing them to share …show more content…
coli with pGLO plasmid that codes for Green Fluorescent Protein (GFP). The pGLO plasmid encodes a fluorescent protein that glows green when exposed to ultraviolet light. Following transformation, the bacteria express their newly acquired gene and produce fluorescent protein which causes them to glow brilliant green under ultraviolet light. The pGLO plasmid also contains an arabinose promoter that regulates the expression of GFP gene. The promoter determines whether the gene will be turned on or off. When arabinose is present in the growth medium, the gene is expressed. However, when arabinose is absent from the growth medium, the green protein gene is not