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The nitrate test was conducted in order to see if nitrate was present. After the addition of two percent acetic acid the unknown had no color change (negative) and therefore the test could not be used for Pseudomonas species. In order to see if the unknown could use citrate as its sole source of carbon, a citrate test was performed. The media of the citrate test contained bromothymol blue, a pH indicator that would have turned blue if the if the organism had a positive reaction. However, my unknown remained green, the original color of the slant and had no growth of the bacteria. The unknown was also unable to utilize malonate as the sole source of carbon. Once the malonate test was done, there was a yellow color, which indicated that it was