Agar Media

General purpose
pattern of hemolysis can aid in ID

blood was heated to lysis to release nutrients
-Haemophilus and Neisseria

(selective chocolate agar) Has antibiotics and selects for N. gonorroeae

High salt con'c
-inhibits most organisms except S. aureus
-fermentation changes from red to yellow

Selective for gram -
(bile salts + crystal violet inhibits gram + and some fastidious -)
-Lactose is sole carb, fermention changes to pink/red, if not clear

similar to MacConkey (except different dyes)
-lactose fermenters changes to blue/black w/ metallic sheen, if not clear

bile salts inhibit gram + and most streptococci except Group D streptococci and enterococcus
<esculin can be hydrolyzed to esculitin which interacts w Fe to produce black precipitate>

selective + differential for gram - (bile salts and indicator dyes inhibit gram +)
-sugars: lactose, sucrose, and salicin) ferments into yellow-pink, non is green/transparent
-if H2S is produced (salmonella) black precipitate

selective and differential for Pseudomonas aeruginosa, inhibits most bacteria as a detergent
-pigments: pyocyanin (blue-green), fluroescein (yello-green)

selective and differential for corynebacterium diptheriae
-K tellurite inhibits gram - and most upper respiratory flora
-colonies appear gray-black (tellurite reductase activity), with brown halo (cystinase activity)

selective and differential for lots of bacteria (based on color; used to identify species or phenotypes -specific and quicker growth