bimm lecture 17

1. adsorption and injection
2. expression of viral early proteins
3. replication of viral DNA expression of viral late proteins
4. assembly
5. lysis and release

Plus strand – the dna that is needed for the virus
Minus strand – the template dna for the plus strand

• dsRNA virus
• body has an immune response to long double stranded rna, body shuts down the translation and kils it.
• In order for this to work, the ras protein needs to have off.
• Ras protein is on in tumor cells.

• RAS:
on with GTP, then promotes cell growth
• GDP is inactivated form
• Nf1 is a GAP, it regulates GTPase activity.
o Can pick up a mutation in ras that makes it stuck
• Immune response to dsRNA is taken out when RAS is activated.

Rabies- ssRNA virus (- strand virus)
• Brain gets degraded by this virus. Kills all the cells in your brain.Uses membrane to leave the cell

- strand ssRNA
acute respiratory infection
virus genome: 8 separate ssRNAs, super mutagenic
genome reassortment: cross species,

dsRNA activated caspase oligomerizers
1 domain recognizes long dsRNA
induces cell suicide in cells infected with any dsRNA, gets rid of cold virus, flus, polio, dengue
how would it work against single strand rna?
Cuz it gets doublestranded at some point

• Fusion, RT, transport to nucleus + integration, transcription, budding
• Proteins on the outside of the virus will recognize cell proteins, fuse
o In the cytoplasm it begins to come apart for RT to begin to copy.
o Primer is a cellular t-RNA to base pair with the sequences in the RNA genome.
o Viral dna is taken by another protein and transported into the nucelous of the cell.
o Integrase – randomly integrated into the genome, much like transposon.
• Called provirus, uses host cell machinery to transcribe to make mRNA’s
• Translated into proteins tha the virus makes, and some of the rna’s will serve as the genome.
 Bud out into other cells and infect them.
• 10kb, 9 genes, 19 different gene products via alt splicing
• 1. Make messenger RNA. Uses full splicing, ligates.
o Rev protein is a protein that goes back into the nucleus. It can bind to a sequence within the retroviral dna. (rev response element in mRNA, inhibits splicing)