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9 Cards in this Set
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Give three examples of post-transcriptional control of gene expression.
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1)Affect mRNA stability 2)Binding of translational inhibitory proteins to UTR of mRNA.
3)Binding of micro-RNAs to UTR of mRNA |
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What are two examples of post-translational regulation of protein activity?
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1)Phosphorylation.
2)GTP loading. |
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What molecular techniques are used to assess differential gene expression/activity?
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1)Northern blot 2)Western blot 3)Microarray
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What techniques are used to obtain spatial information about gene expression?
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1)In situ hybridization 2)Immunocytochemistry
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What techniques allow you to assess the function of regulatory DNA elements?
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1.Reporter assay 2.Enhancer trap 3.Promoter bashing
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What is the receptor in the Wnt/beta-catenin pathway?
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Frizzled
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What are the Co-receptors in the Wnt/beta-catenin pathway?
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1.Lrp - LDL-receptor related protein
2.Glypicans – sulfated proteoglycans |
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What are the Intracellular factors in the Wnt/beta-catenin pathway?
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1)APC/Axin/GSK-3 2)Dishevelled 3)Beta-catenin
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What experiment gave evidence that a gradient is required for pattern formation?
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Klaus Sander tied strings around drosophila embryos. No middle segments formed (anterior and posterior segments developed normally)
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