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10 Cards in this Set
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- Back
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Lac operon
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codes for 3 prots involved in the catabolism of the disaccharide lactose
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LacZ codes for what?
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Beta-galactosidase, which hydrolyzes lactose to galactose and glucose
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LacY gene codes for what?
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permease that facilitates the movement of lactose into the cell
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LacA gene codes for what?
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galactoside transacetylase, non metabolites removal
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What does glucose do in catabolite repression?
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glucose reduces cAMP which is required for activation of CAP (DNA binding prot) and transcription of lac operon
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High glucose = ?
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low cAMP
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Low glucose = ?
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high cAMP = active lac operon
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E.coli regulation is at what level?
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regulation at the level of transcription
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Operons
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concentrate functionally related genes in a sequence in the chromosome (not so in eukaryotes)
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How do DNA binding prots work?
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positive cntrl (CAP-cAMP) and negative cntrl (lac and trp repressor)
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