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8 Cards in this Set
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- Back
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Agarose gel eletrophoresis separates DNA fragments based on what?
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Size - smaller travels further
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What is labeled on a Southern blot?
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DNA fragments
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What is labeled on a Northern blot?
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RNA fragments
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What is labeled on a Western blot?
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Proteins
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What is labeled on a Southwestern blot?
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DNA-binding proteins (e.g. transcription factors)
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How does ELISA work?
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Patient blood is probed with a test antigen or test antibody coupled to a color-generating enzyme activated if the reactive antigen or antibody is present
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What is the cre-lox system?
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Inducibly manipulates genes at developmental points with antibiotic-controlled promotor
Allows study of genes whose deletion --> death |
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What is RNAi?
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dsRNA that is complementary to the target mRNA sequence; transfection into cells promotes degradation of the target mRNA, lowering gene expression
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