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35 Cards in this Set
- Front
- Back
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types of basic nutrient media
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nutrient agar
trypticase soy agar |
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types of enriched media
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blood agar plate
chocolate agar |
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types of selective media
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phenylethel alcohol agar
macconkey agar |
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types of differential media
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macconkey
hektoen enteric agar triple sugar iron |
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types of selective media broth
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selenite broth
tetrathionate broth |
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misc types of media
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brain heart infusion broth
motility media indole test media |
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basic nutrient media definition
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general all purpose media. supports growth of most bacterial. used in high volume lab.
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nutrient agar definition
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good for the growth of less fastidious bacteria
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trypticase soy agar definition
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supports groth of most clinical bacteria
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enriched media definition
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basic nutrient media with extra nutrients. usually blood, serum, or egg. for fastidious bacteria
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blood agar plate definition
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very common. contains 5% sheeps blood in the trypticase soy agar.
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chocolate agar definition
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blood is added to nutrient agar & then heated used to grow STD's (brucella)
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definition of selective media
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contains a substance that selects 1 type of bacteria and inhibits other bacterial growth. may contain bile salt, abx, etc.
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definition phenylethel alcohol agar
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selects gram positive bacteria only. inhibits gram negative bacteria.
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definition of macconkey agar
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pretty popular. selects gram negative rods only. inhibits all other bacteria types.
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definition of differential media
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selects for a group of bacteria and contains an indicator that visibly is changed by metabolic activity of the bacteria.
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definition of macconkey agar
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colonies change color if bacteria can ferment lactose. fermenters are pink. non fermenters are clear.
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definition of hektoen enteric agar
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selects gram negative enteric pathogens. inhibits all normal flora. good for salmonella. fermenter= salmon. non fermenters are blue green.
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definitionj of triple sugar iron agar
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used to classify bacteria by ability to ferment. yellow= ferm. red=no ferm.
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definition of mannitol salt agar
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selects for g. staphylococci. can be used to isolate staph arreus. aureus= eyllow. non path= red
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defintion of selective media broths
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used for fecal cultures
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definition of selenite broth
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used for feces of non ruminants
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definition of tetrathionate broth
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suppresses coloform sp for ruminants
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definition of brain heart infusion broth
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useful all purpose broth. used to increase number of organisms before plating
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definition of motility media
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find out if bacteria are mobile or not.
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definition of indole test media
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compound produced by tripifan de comp in intestines. gives feces its odor. tests for the ability of bacteria to produce indole.
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alpha
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alpha hemolysis is partial hemolysis that creates a narrow band of greenish slime around bacteria colony.
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beta
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complete hemolysis. creates a clear zone around bacteria colony
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gamma
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hemolysis that produces no change in appearance of medium
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delta
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zone of hemolysis is surrounded by a narrow zone of hemolysis around bacteria colony.
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materials needed for innoculation
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medium/media
culture loop wire incubator flame/lighter |
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initial plating technique
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protocol
contamination application |
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how long do you incubate media
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24-48hrs
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colony characteristics
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size- pinpoint med or large
color - transparent or opaque density - elevation form consistency odor hemolysis - breaking down of blood cells (A, B, G, D) |
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sensitivity testing types
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kirby bauer
minimum inhibitory concentration |
