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22 Cards in this Set
- Front
- Back
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Who is the father of microbiology?
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Anton van Leeuwenhoek
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What's the formula for resolving power?
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resolving power = wavelength/2xnumerical aperture
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In which type of microscopy is the object brightly illuminated and the specimen appears in its natural color or the color it has been stained?
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Bright Field
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Which type of microscopy utilizes a special condenser that blocks the light and the specimen appears brightly illuminated against a black background and you see a reflection of the specimen?
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Dark Field
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Which type of microscopy uses uses UV light and fluorescent dyes?
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Fluorescent
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Which type is used to resolve objects smaller than .16 microns?
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Ultraviolet (325 nm)
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UV light _______(can/can't) be seen with the human eye?
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Can't
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UV light microscopy must be recorded on a ________ plate.
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Photographic
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Which type of microscopy utilizes visible light and a special condenser which sends light to a phase shifting plate?
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Phase Contrast
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Phase Contrast makes __________ more visible.
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Structural details
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Which type of microscope uses electron beams in a magnetic field and magnifies 100,000 - 200,000x?
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Electron microscope
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Light microscopes can resolve objects with diameters of ____ microns
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.2
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How do you get total magnification of a microscope?
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magnifying power of the objective is multiplied by the magnifying power of the ocular
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1.25 is the _______ for oil.
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Numerical Aperture
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Low Power Objective magnifies ___ for a total of _____.
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10 times for 100X
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Hi-dry magnification magnifies ___ for a total of ______.
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40 times for 400x
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Oil Immersion magnifies ____ for a total of ______.
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100 times for 1000X
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LOW POWER
Field of Vision:____ Focal Distance: ____ |
1.8 mm
16 mm |
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Hi-Dry Power
Field of vision: ____ Focal Distance: ____ |
450 microns
4 mm |
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Oil
Field of vision: ____ Focal Distance: ____ |
180 microns
1.8 mm |
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What does parfocal mean?
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It means only fine adjustments are needed when changing from low power to higher power objective if coarse adjustment is set.
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Immersion oil has the same ____________ as glass, and its use ______( reduces/increases) the loss of light that occurs by refraction at glass-air interfaces.
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refractive index
reduces |
