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184 Cards in this Set

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Acid-fast
Resistance to destaining by mild acids; a property exhibited by mycobacteria and some actinomycetes. [p.23, 28-29]
Acidophile
Grows in low pH (less than 3) [68, 299]
Actinomycytes
A large group of gram-positive bacteria capable of differentiation into aerial hyphae and spores; producers of many antibiotics. [174, 309-310]
Aerobic
In the presence of O2. Describes organisms that require O2 for growth or growth conditions with O2. [99]
Aerobic Respiration
Respiration in which O2 gas is the terminal electron acceptor. [92-92, 95, 378]
Alkaliphiles
Organisms that thrive in alkaline environments (pH greater than 10). [68]
Anabaena
Photoautotroph [379]
Anaerobic respiration
Respiratory process with a terminal electron acceptor other than O2. [92-95, 240, 300, 356-357]
Anaerobic
In the absence of O2. Used to describe an organism that is sensitive to O2 or that requires growth conditions without O2. [99, 356]
Antigen
Molecule recognized by the immune system.
Apoptosis
Cell death due to an intracellular developmental program or induced by other cells or infectious agents. [180]
Archaea
Prokaryotes; Share basic morphology with bacteria; resemble Eukaryotes in molecular details (Histone bound DNA) [4, 29, 30-31, 43, 132, 141-142, 174,
ATP
Adenosine triphosphate: The energy tranfer molecule of life. Adenine-ribose-triphosphate compund that conserves metabolic energy in its two high-energy phosphate bonds. [72, 76, 85]
Autotroph
An organism that derives all its carbon from CO2 + inorganic energy source. (eg. Light, thermal vents, etc) [46, 75, 78-80, 86, 95, 356-357]
Bacillus
Rod shaped bacteria. [24]
Bacterial capsule: definition and compostition
Diffuse, outermost layer surrounding microbes composed of complex polysaccharides (usually carbohydrate). [31-32]
Balanced growth
The condition of growth in which all cell constituents increase by the same factor over a period of time. Usually synonymous with exponential or steady-state growth. [57-58]
Cell wall: Composition
Peptidoglycan [20, 23-26, 30]
Chemoautotroph
use inorganic energy sources, such as hydrogen sulfide, elemental sulfur, ferrous iron, molecular hydrogen, and ammonia. [46, 52, 79, 85, 93-94, 362, 368]
Chemoheterotroph
Derives its ATP from energy obtained by oxidizing inorganic nutrients, such as ferrous ion, hydrogen sulfide, or hydrogen gas. [79, 356]
Chemotroph
organisms that obtain energy by the oxidation of electron donors in their environments. These molecules can be organic (chemoorganotrophs) or inorganic (chemolithotrophs). The chemotroph designation is in contrast to phototrophs, which utilize solar energy. Chemotrophs can be either autotrophic or heterotrophic.
Chlorobium tepidum
Photoautotroph
Cilia
Organelle of eukaryotic protists, the ciliates, serving as a sense organ or means of motility. [320-322]
Coccus
Spherical bacteria. [24]
Crystalline surface layer
S-Layer. Surface layer of some bacteria and archeae consisting of protein arrays, usually resistant to chemicals and proteases. [29-30]
Cytoplasm: composition
Proteins; tRNAs; Glycogen; various inclusions [39, 41, 44-45]
Describe the layers of Gram-negative bacteria envelope.
Three layers: Inner cell membrane; Periplasm containing a thin cell wall composed of murein; Outer cell membrane with outer leaflet of Lipopolysaccharide.
Describe the layers of Gram-positive bacteria envelope.
Two layers: Cell membrane surrounded by a thick cell wall composed of murein with teichoic acid and lipoteichoic acid protrusions.
Escherichia coli
Chemoheterotroph [27, 43]
Eukaryotes
Membrane bound organelles; Respiration on mitochondria; DNA contained with membrane of nucleus; Some have cell walls; Histone bound DNA [3, 5, 86]
Example of bacillus
E. Coli; causes food poisening; gram negative.
Example of coccus
Streptococcus pneumoniae: Causes pneumonia, Gram-positive
Example of spirillum
Treponema pallidum; Causes syphilis
Flagella: Types and composition
Single polar, tuft (multi polar), all around (peritrichous); Protein [31-36, 263-267]
Glycerol teichoic acid
Molecule of glycerol linked by phosphate groups in gram-positive bacteria walls. [26]
Gram-negative
Envelope composed of an outer membrane surrounding a thin peptidoglycan layer. Stains purple with crystal violet stain followed by the addition of iodine as a trapping agent. [23-36]
Gram-positive
Envelope composed of a thick peptidoglycan layer and no outer membrane. Stains pink with safranin binding to the thick murein (peptidoglycan) layer of the cell wall. [23-36]
Gram-stain
Method of determining some aspects of the chemical and physical structure of bacterial cell walls; two large groups (Gram-positive and Gram-negative); Primarily, it detects peptidoglycan, which is present in a thick layer in Gram positive bacteria.[1] A Gram positive results in a purple/blue color while a Gram negative results in a pink/red color. [23]
Halophile
Grows in the presence of high salt consentrations. [300-302]
Heterotroph
Utilizes organic nutrients as a source of carbon and energy; aka Organotroph [74-89, 98-108]
Hyperthermophile
Grows in extremely high temperatures [62-63]
Isoelectric point
The pH at which a molecule carries no net charge. [253-254]
Kinetoplast
specialized mitochondria at the base of the cilium in ciliates. [380-381]
Lactobacillus
Chemoheterotroph [89, 305]
Lipid A
Phosphoralated glycolipid common to all bacterial lipopolysaccharides [26-27, 126-127, 165]
Lipopolysaccharide
A major component of the outer layer of the outer membrane in gram-negative bacteria. [24-27]
Lipoprotein
Protein containing covalently bound fatty acids. [126-127, 160, 165]
Lipoteichoic acid
major constituent of the cell wall of Gram-positive bacteria; Involved in immune response and apoptosis. [24]
Lithotroph
aka. Chemoautotroph [79]
Log phase
Phase of growth cycle when growth is exponential; aka Exponential phase
LPS
Lipopolysaccharide
Lysozyme
Enzyme that hydrolyzes murein
Murien
The form of peptidoglycan found in the walls of bacteria. [24-29, 299]
Mycobacteria
Classified as gram-positive, these bacteria have features of both gram-negative and positive bacteria. The cell membrane is covered with a peptidoglycan layer, then arabinogalactan and mycolic acid layers and topped off with a capsule-like material. These features inhibit the macrophage immunity response to infection such apoptosis and secretion of inflammatory cytokines.
Nitrobacter sp.
Chemoautotroph [209]
Nuclease
An enzyme that cleaves phosphate-deoxyribose bonds within (endonuclease) or at the end of (exonuclease) a nucleotide sequence.
Nucleoid
condensed organization of a prokayotic chromosome within the cell. [39-44, 298]
Open reading frame
Stretch of DNA that potentially codes for protein. ORF
Operon
Sequence of adjacent genes read as a single, polycistronic messenger RNA. Changes in the level of transcription thus affect all of the genes in an operon, so genes are often coordinately regulated.
Organotroph
Heterotroph
Outer membrane
Outermost lipid bilayer of gram-negative bacteria, consisting of an outer lipopolysaccharide leaflet and an inner phospholipid leaflet plus proteins. [24-27, 158, 165, 99-100]
Outer membrane; composition
Proteins; Phospholipids; Lipopolysaccharide
Periplasm
Space between inner and outer cell membranes of a gram-negative bacteria. Composed of proteins. [24-28, 99, 158]
Photoautotroph
Derive ATP and reducing power from light energy and carbon from CO2 [52, 79, 93, 306, 362]
Photoheterotroph
use light for energy, but cannot use carbon dioxide as their sole carbon source. Consequently, they use organic compounds from the environment to satisfy their carbon requirements. They use compounds such as carbohydrates, fatty acids and alcohols as their organic "food". Examples are purple non-sulfur bacteria, green non-sulfur bacteria and heliobacteria. [79, 94, 96]
Phototroph
Generates ATP and reducing power from light energy. [78]
Pili
Filaments that extend from the microbial cell surface to facilitate adhesion to surfces and other cells. ; Composittion: Proteins [31-37, 132, 167]
Polysome
aka Polyribosome; Messenger RNA molecule being transcribed by more than one ribosome. ; Composition: mRNA, Ribosomes: rRNAs; riboproteins [23, 40, 44, 153-154]
Porins
Channels formed by protein triads in the outer cell membranes of gram-negative bacteria, slowing nonspecific diffusion of hydrophilic compounds, such as sugars, amino acids, and some ions. [24-28, 99, 241, 252]
Prokaryotes
Lack membrane bound organelles; Respirate at cell membrane; DNA concentrated in nucleoid region; Have cell walls; Exchange genetic information with plasmids
Protease
An enzyme that degrades protein.
Protoplasts
A cell bound by the cytoplasmic membrane and a portion (usually unknown) of the envelope material exterior to it. if significant amounts of envelope remain, the structure is known as a Spheroplasts. [25-26]
Pseudomurein
Form of peptidoglycan found in the cell walls of some archaea. [31, 299]
Quorum sensing
Chemical process by which populations of microbes sense their density as a result of each cell secreting a small amount of a certain compound. [268-271, 280]
Reading frame
The way in which a sequence of nucleotides that code for a polypeptide are read as consecutive triplets.
Rhodobacter capsulatus
Photoautotroph [95]
Ribitol teichoic acid
Ribitol units linked by phosphate groups in gram-positive bacteria walls. [26]
Sacculus
Murein (peptidoglycan) bag holding a bacteria. [24-25, 31, 164]
Selective medium
Growth medium that allows growth of only certain species, stains, or mutants. [52]
S-layer
Crystalline surface layer [29-31]
Slime layer
Thin slimy or gummy layer surrounding many prokaryotic cells [31-32, 267]
Spheroplasts
Osmotically sensitive cell whose cell wall has benn partially removed; aka protoplast [25, 30]
Spirillum
Spiral shaped bacteria. [24]
Teichoic acid
Molecule composed of glycerol or ribitol units linked by phosphate groups; Found in walls of gram-positive bacteria [24, 26, 34]
Thermoacidophile
Grows in high temperature low pH. [9-10, 62-65, 299]
Thermophile
Grows in high temperature. [9-10, 62-65, 299]
Three Domains
Bacteria; Archaea; Eukarya
Vesicles: composistion
Protein; phospholipid membranes
Viability
Ability to grow and divide [360-361]
Viable count
Determination of the number of living cells in a population, usually carried out by placing aliquots of dilutions on agar plates and counting the resulting colonies. [54]
β-lactamase
An enzyme that cleaves the B-lactam ring of B-lactam antibiotics, thus inactivating the antibiotics. The ampicillin resistance encoded by many common plasmids is due to a secreted B-lactamase
Autoclave
generate steam (at 121C) under pressure to sterilize.
Acid-fast
Resistance to destaining by mild acids; a property exhibited by mycobacteria and some actinomycetes. [p.23, 28-29]
Acidophile
Grows in low pH (less than 3) [68, 299]
Actinomycytes
A large group of gram-positive bacteria capable of differentiation into aerial hyphae and spores; producers of many antibiotics. [174, 309-310]
Aerobic
In the presence of O2. Describes organisms that require O2 for growth or growth conditions with O2. [99]
Aerobic Respiration
Respiration in which O2 gas is the terminal electron acceptor. [92-92, 95, 378]
Alkaliphiles
Organisms that thrive in alkaline environments (pH greater than 10). [68]
Anabaena
Photoautotroph [379]
Anaerobic respiration
Respiratory process with a terminal electron acceptor other than O2. [92-95, 240, 300, 356-357]
Anaerobic
In the absence of O2. Used to describe an organism that is sensitive to O2 or that requires growth conditions without O2. [99, 356]
Antigen
Molecule recognized by the immune system.
Apoptosis
Cell death due to an intracellular developmental program or induced by other cells or infectious agents. [180]
Archaea
Prokaryotes; Share basic morphology with bacteria; resemble Eukaryotes in molecular details (Histone bound DNA) [4, 29, 30-31, 43, 132, 141-142, 174,
ATP
Adenosine triphosphate: The energy tranfer molecule of life. Adenine-ribose-triphosphate compund that conserves metabolic energy in its two high-energy phosphate bonds. [72, 76, 85]
Autotroph
An organism that derives all its carbon from CO2 + inorganic energy source. (eg. Light, thermal vents, etc) [46, 75, 78-80, 86, 95, 356-357]
Bacillus
Rod shaped bacteria. [24]
Bacterial capsule: definition and compostition
Diffuse, outermost layer surrounding microbes composed of complex polysaccharides (usually carbohydrate). [31-32]
Balanced growth
The condition of growth in which all cell constituents increase by the same factor over a period of time. Usually synonymous with exponential or steady-state growth. [57-58]
Cell wall: Composition
Peptidoglycan [20, 23-26, 30]
Chemoautotroph
use inorganic energy sources, such as hydrogen sulfide, elemental sulfur, ferrous iron, molecular hydrogen, and ammonia. [46, 52, 79, 85, 93-94, 362, 368]
Chemoheterotroph
Derives its ATP from energy obtained by oxidizing inorganic nutrients, such as ferrous ion, hydrogen sulfide, or hydrogen gas. [79, 356]
Chemotroph
organisms that obtain energy by the oxidation of electron donors in their environments. These molecules can be organic (chemoorganotrophs) or inorganic (chemolithotrophs). The chemotroph designation is in contrast to phototrophs, which utilize solar energy. Chemotrophs can be either autotrophic or heterotrophic.
Chlorobium tepidum
Photoautotroph
Cilia
Organelle of eukaryotic protists, the ciliates, serving as a sense organ or means of motility. [320-322]
Coccus
Spherical bacteria. [24]
Crystalline surface layer
S-Layer. Surface layer of some bacteria and archeae consisting of protein arrays, usually resistant to chemicals and proteases. [29-30]
Cytoplasm: composition
Proteins; tRNAs; Glycogen; various inclusions [39, 41, 44-45]
Describe the layers of Gram-negative bacteria envelope.
Three layers: Inner cell membrane; Periplasm containing a thin cell wall composed of murein; Outer cell membrane with outer leaflet of Lipopolysaccharide.
Describe the layers of Gram-positive bacteria envelope.
Two layers: Cell membrane surrounded by a thick cell wall composed of murein with teichoic acid and lipoteichoic acid protrusions.
Escherichia coli
Chemoheterotroph [27, 43]
Eukaryotes
Membrane bound organelles; Respiration on mitochondria; DNA contained with membrane of nucleus; Some have cell walls; Histone bound DNA [3, 5, 86]
Example of bacillus
E. Coli; causes food poisening; gram negative.
Example of coccus
Streptococcus pneumoniae: Causes pneumonia, Gram-positive
Example of spirillum
Treponema pallidum; Causes syphilis
Flagella: Types and composition
Single, tuft, all around(find better term); Protein [31-36, 263-267]
Glycerol teichoic acid
Molecule of glycerol linked by phosphate groups in gram-positive bacteria walls. [26]
Gram-negative
Envelope composed of an outer membrane surrounding a thin peptidoglycan layer. Stains purple with crystal violet stain followed by the addition of iodine as a trapping agent. [23-36]
Gram-positive
Envelope composed of a thick peptidoglycan layer and no outer membrane. Stains pink with safranin binding to the thick murein (peptidoglycan) layer of the cell wall. [23-36]
Gram-stain
Method of determining some aspects of the chemical and physical structure of bacterial cell walls; two large groups (Gram-positive and Gram-negative); Primarily, it detects peptidoglycan, which is present in a thick layer in Gram positive bacteria.[1] A Gram positive results in a purple/blue color while a Gram negative results in a pink/red color. [23]
Halophile
Grows in the presence of high salt consentrations. [300-302]
Heterotroph
Utilizes organic nutrients as a source of carbon and energy; aka Organotroph [74-89, 98-108]
Hyperthermophile
Grows in extremely high temperatures [62-63]
Isoelectric point
The pH at which a molecule carries no net charge. [253-254]
Kinetoplast
specialized mitochondria at the base of the cilium in ciliates. [380-381]
Lactobacillus
Chemoheterotroph [89, 305]
Lipid A
Phosphoralated glycolipid common to all bacterial lipopolysaccharides [26-27, 126-127, 165]
Lipopolysaccharide
A major component of the outer layer of the outer membrane in gram-negative bacteria. [24-27]
Lipoprotein
Protein containing covalently bound fatty acids. [126-127, 160, 165]
Lipoteichoic acid
major constituent of the cell wall of Gram-positive bacteria; Involved in immune response and apoptosis. [24]
Lithotroph
aka. Chemoautotroph [79]
Log phase
Phase of growth cycle when growth is exponential; aka Exponential phase
LPS
Lipopolysaccharide
Lysozyme
Enzyme that hydrolyzes murein
Murien
The form of peptidoglycan found in the walls of bacteria. [24-29, 299]
Mycobacteria
Classified as gram-positive, these bacteria have features of both gram-negative and positive bacteria. The cell membrane is covered with a peptidoglycan layer, then arabinogalactan and mycolic acid layers and topped off with a capsule-like material. These features inhibit the macrophage immunity response to infection such apoptosis and secretion of inflammatory cytokines.
Nitrobacter sp.
Chemoautotroph [209]
Nuclease
An enzyme that cleaves phosphate-deoxyribose bonds within (endonuclease) or at the end of (exonuclease) a nucleotide sequence.
Nucleoid
condensed organization of a prokayotic chromosome within the cell. [39-44, 298]
Open reading frame
Stretch of DNA that potentially codes for protein. ORF
Operon
Sequence of adjacent genes read as a single, polycistronic messenger RNA. Changes in the level of transcription thus affect all of the genes in an operon, so genes are often coordinately regulated.
Organotroph
Heterotroph
Outer membrane
Outermost lipid bilayer of gram-negative bacteria, consisting of an outer lipopolysaccharide leaflet and an inner phospholipid leaflet plus proteins. [24-27, 158, 165, 99-100]
Outer membrane; composition
Proteins; Phospholipids; Lipopolysaccharide
Periplasm
Space between inner and outer cell membranes of a gram-negative bacteria. Composed of proteins. [24-28, 99, 158]
Photoautotroph
Derive ATP and reducing power from light energy and carbon from CO2 [52, 79, 93, 306, 362]
Photoheterotroph
use light for energy, but cannot use carbon dioxide as their sole carbon source. Consequently, they use organic compounds from the environment to satisfy their carbon requirements. They use compounds such as carbohydrates, fatty acids and alcohols as their organic "food". Examples are purple non-sulfur bacteria, green non-sulfur bacteria and heliobacteria. [79, 94, 96]
Phototroph
Generates ATP and reducing power from light energy. [78]
Pili
Filaments that extend from the microbial cell surface to facilitate adhesion to surfces and other cells. ; Composittion: Proteins [31-37, 132, 167]
Polysome
aka Polyribosome; Messenger RNA molecule being transcribed by more than one ribosome. ; Composition: mRNA, Ribosomes: rRNAs; riboproteins [23, 40, 44, 153-154]
Porins
Channels formed by protein triads in the outer cell membranes of gram-negative bacteria, slowing nonspecific diffusion of hydrophilic compounds, such as sugars, amino acids, and some ions. [24-28, 99, 241, 252]
Prokaryotes
Lack membrane bound organelles; Respirate at cell membrane; DNA concentrated in nucleoid region; Have cell walls; Exchange genetic information with plasmids
Protease
An enzyme that degrades protein.
Protoplasts
A cell bound by the cytoplasmic membrane and a portion (usually unknown) of the envelope material exterior to it. if significant amounts of envelope remain, the structure is known as a Spheroplasts. [25-26]
Pseudomurein
Form of peptidoglycan found in the cell walls of some archaea. [31, 299]
Quorum sensing
Chemical process by which populations of microbes sense their density as a result of each cell secreting a small amount of a certain compound. [268-271, 280]
Reading frame
The way in which a sequence of nucleotides that code for a polypeptide are read as consecutive triplets.
Rhodobacter capsulatus
Photoautotroph [95]
Ribitol teichoic acid
Ribitol units linked by phosphate groups in gram-positive bacteria walls. [26]
Sacculus
Murein (peptidoglycan) bag holding a bacteria. [24-25, 31, 164]
Selective medium
Growth medium that allows growth of only certain species, stains, or mutants. [52]
S-layer
Crystalline surface layer [29-31]
Slime layer
Thin slimy or gummy layer surrounding many prokaryotic cells [31-32, 267]
Spheroplasts
Osmotically sensitive cell whose cell wall has benn partially removed; aka protoplast [25, 30]
Spirillum
Spiral shaped bacteria. [24]
Teichoic acid
Molecule composed of glycerol or ribitol units linked by phosphate groups; Found in walls of gram-positive bacteria [24, 26, 34]
Thermoacidophile
Grows in high temperature low pH. [9-10, 62-65, 299]
Thermophile
Grows in high temperature. [9-10, 62-65, 299]
Three Domains
Bacteria; Archaea; Eukarya
Vesicles: composistion
Protein; phospholipid membranes
Viability
Ability to grow and divide [360-361]
Viable count
Determination of the number of living cells in a population, usually carried out by placing aliquots of dilutions on agar plates and counting the resulting colonies. [54]
β-lactamase
An enzyme that cleaves the B-lactam ring of B-lactam antibiotics, thus inactivating the antibiotics. The ampicillin resistance encoded by many common plasmids is due to a secreted B-lactamase
Autoclave
generate steam (at 121C) under pressure to sterilize.