Microbiology Lab #2 study questions

By aback85, Sep 2008

Front

Right/Left arrow keys: Navigate between flashcards.right arrow keyleft arrow key

Up/Down arrow keys: Flip the card between the front and back.down keyup key

H key: Show hint (3rd side).h key

PLAY BUTTON

Progress

1/10

Click to flip

Define "aeseptic technique"	Techniques that aim to prevent contamination.
What instrument is used to inoculate each of these? 1. broth culture 2. deep 3. petri plate medium 4. slant	1. loop 2. needle 3. loop 4. loop
When streaking for isolation: streak #1 is followed by flaming the loop. Do you place more bacteria on the slide for streak #2?	No. Only place loop in bacterial broth once.
Name the organism used in the lab to inoculate the broth, slant, and deep.	Serratia marcescens.
What is the difference in procedure when preparing a bacterial smear on a slide from a broth vs. a solid culture?	Broth- use no water. Solid- place water on slide first.
What is the purpose of heat-fixing the bacterial smear?	It denatures the bacteria so it sticks better to the slide.
Is the dye Methylene Blue acidic or basic?	Basic (positively charged). The bacteria is negatively charged so a basic dye will attract better to it.
Why is the negative staining procedure better than the simple stain when observing morphologies?	The negative stain does not stain the bacteria, but only the surrounding area. Dyeing the bacteria could alter the morphology.
What dye was used in the simple stain?	Methylene Blue.
What dye was used in the negative stain?	Nigrosen.

Browse Flashcards