Microbiology Lab #4 and Lab #5 up to page 45 Flash Cards

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Title: Microbiology Lab #4 and Lab #5 up to page 45
Description: Microbiology Lab 4
Number of Cards: 24
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Author: mikesperry799
Created: 2012-01-26
Tags: microbiology
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    • Question
    • Answer
    • Side 3
    • What is the proper way to label Agar Plates?
    • Organism Name
      Media, Temperature
      Date, Lab#
      Your Name
    • Organisms that like cool temperatures (4C).
    • Psychrophiles
    • Organisms that like room temperature (25C) or body temperature (37C).
    • Mesophiles
    • Organisms that like warm environment (55-60C).
    • Thermophiles
    • What effect can salt have on a microorganism.
    • Salt and sugar are both known to change the osmotic pressure of a microbe.
    • Organisms that are very salt tolerant.
    • Halophiles
    • Organisms that like pH of 8 or greater.
    • Alkaliphiles
    • Organisms that prefer pH below 5.
    • Acidiphiles
    • Examples of UV resistant microorganism.
    • Deinococcus radiodurans
      Micrococcus luteus
    • Example of organism used to demonstrate mutations UV can cause at various lengths of exposure.
    • Serratia marcescens
    • What is "Tube Quantification"?
    • Method of quantifying bacteria growth based on turbidity within test broth tube.
    • Require oxygen in order to live and reproduce
    • Aerobes
    • List and define the two types of aerobes discussed in lab.
    • 1.) Strict (obligate)- require oxygen to survive and maintain metabolic pathways
      2.) Microaerophilic- prefer less oxygen than what is present in the atmosphere.
    • Do not require oxygen for growth.
    • Anaerobes.
    • List and define the two types of anaerobes discussed in lab.
    • 1.) Strict (obligate)- cannot live in presence of oxygen.
      2.) Facultative: can live in the presence or absence of oxygen.
    • Cannot use oxygen, but can live in the presence of oxygen.
    • Aerotolerant
    • Define Superoxide Dismutase.
    • Breaks down oxygen radical into hydrogen peroxide

      2O2- + 2H+ -> O2 + H2O2
    • Define Catalase.
    • Breaks down hydrogen peroxide into water and non-toxic oxygen.

      2(H2O2) -> 2(H2O) + O2
    • Example of strict aerobe.
    • Mycobacterium leprae
      Mycobacterium tuberculosis
      Pseudomonas aeruginosa
    • Example of microaerophilic organisms.
    • Campylobacter jejuni
      Neisseria gonorrohoeae
      Micrococcus luteus
    • Examples of an aerotolerant microbe.
    • Propionibacterium acnes
    • Give examples of Facultative anaerobe.
    • Staphylococcus aureus
      Streptococcus pyogenes
    • Give examples of strict anaerobe.
    • Clostridium botulinum
      Bacteroides fragilis
    • What is an anaerobe jar and how does it work?
    • Jar that we can use to obtain completely oxygen free environment.
      1.) outer and inner lid with gasket
      2.) catalyst chamber in lid or gas pan contain palladium crystals to provide place for hydrogen to bind with oxygen to form water.
      3.) gas pan generator contains sodium bicarbonate so when you add water, hydrogen and carbon dioxide are formed
      4.) an indicator strip containing methylene blue is used to confirm the absence of oxygen. The strip is blue when oxygen is present, but will turn colorless as all of the oxygen is used.