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52 Cards in this Set

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WBC Normal Range
4.4-11.3 x 103 cells/!L
Neutrophils (PMNs) Normal Range
45-73%
Increased PMN Means...
bacteria, fungi
Band neutrophils Normal Range
3-5%

"left shift"
Lymphocytes Normal Range
20-40%
Increased Lymphocytes means...
viral infections
Monocytes Normal Range
2-8%
Eosinophils Normal Range
0-4%
Increased Eosinophils means...
bacterial, parasitic infections
Basophils Normal Range
0-1%
Increased Basophils associated with...
allergic response
RBC Male Normal Range
4.5-5.9 x 106 cells/!L
RBC Female Normal Range
4.1-5.1 x 106 cells/!L
MCV Normal Range
80-96%
Hgb Male Normal Range
14-17.5 g/dL
Hgb Female Normal Range
12.3-15.3 g/dL
MCH Normal Range
27-33 pg/cell
Hct Male Normal Range
42 - 50%
Hct Female Normal Range
36 - 45%
Platelets Normal Range
150k - 450k/uL
Specific Gravity of Urine Norm
1.010 - 1.025 mg/mL
pH of Urine Normal
6-8 pH
Urine Protein Normal
2 - 8 mg/dL
Urobilinogen Normal in Urine
0.1-1 unit/dL
Two things that indicate bacterial infection in urine:
Nitrite; Leukocyte esterase
Na+ Normal
135-145 mEq/L
K+ Normal
3.5-5 mEq/L
CO2 Normal
24-30 mEq/L
Cl- Normal
96-106 mEq/L
FBS Normal
80-110 mg/dL
A1C Normal
4-6%
BUN Normal
8 - 20 mg/dL
Cr Normal
0.7-1.5 mg/dL
CrCl
90-140 mL/min/1.73m2
CrCl Formula (Includes IBW)
((140-age) x IBW) / (72 x SCr*) [x 0.85 in females]

IBW = [50gk(males) or 45kg(female)] + 2.3kg (for every inch >5ft)

Round SCr to 1 in frail elderly
AST (SGOT) Normal
8-42 IU/L

increased with alcohol consumption; liver damage
ALT (SGPT) Normal
3-30 IU/L

increased with hepatitis; liver damage
ALP Normal
44-147 IU/L
Total bilirubin Normal
0.3-1 mg/dL
Total protein Normal
5.5-8.3 g/dL
Albumin Normal
3.5-5 g/dL

decreased with cirrhosis; alcoholism; trauma; malnutrition
PT, clotting time Normal
10-13 sec

affected by drugs (Coumadin) and plts

INR = 1
Gram +
pink
Gram -
purple
Acid fast +
red
Acid fast -
blue
Serial two-fold dilutions of antibiotics in Mueller-Hinton broth
Addition of standard inoculum (105) of bacteria.
Broth dilution-Macrodilution
Micro titer plate: serial dilutions of multiple antibiotics
Used to identify ABC,GBS and several others
Cannot be used for H.influenzae, N.gonorrhoea, S. pneumoniae
Panels can be chosen based on each facility antibiogram and formulary.
Broth dilution-Microdilution
Predefined stable gradient of 15 antibiotic concentrations on a tray
E-test
Ppluarset ic uslttruipre of Microorganism is grown overnight then diluted to a
concentration of 1x106 microorganisms per ml
Spread suspension evenly on the sterile plate surface
If a substantial antimicrobial activity is present, a zone of inhibition
appears around the test product
Zone of inhibition-kirby Bauer
Disadvantage of Kirby-Bauer
Does not work for
viruses
Not quantitative, hard to
tell MIC using this
method but in general
larger inhibition zones
correlates with low MIC
Disadvantage of E-test
None