- Shuffle
Toggle OnToggle Off
- Alphabetize
Toggle OnToggle Off
- Front First
Toggle OnToggle Off
- Both Sides
Toggle OnToggle Off
Front
How to study your flashcards.
Right/Left arrow keys: Navigate between flashcards.right arrow keyleft arrow key
Up/Down arrow keys: Flip the card between the front and back.down keyup key
H key: Show hint (3rd side).h key
PLAY BUTTON
PLAY BUTTON
9 Cards in this Set
- Front
- Back
|
PCR:
Linear vs. exponential |
You get exponential growth of the product (cycle^#)
& you get linear growth of the original template (x cycle #) |
|
What is the role of the primers in PCR?
|
Primer anneals to the DNA strand so TAQ polymerase can add on
|
|
What are the PCR components?
|
template DNA, 2 primers (forward & reverse), dNTPs, Polymerase, magnesium, thermocycler
|
|
Tm = ?
|
Tm= (A+T) x 2 + (G+C)x 4
determines the annealing temperature |
|
Primer 1:
Primer 2: |
Primer 1: forward direction, reads like the gene
Primer 2: reverse direction, inversion of the gene |
|
What is real time PCR?
|
“real-time PCR” allows us to actually view the increase in the amount of DNA as it is amplified
|
|
SYBR green dye
|
binds to double stranded DNA, it also increase the fluorescent emissions. so the more DNA the more signal increase
|
|
what is the advantage of using real time PCR?
|
no gel, detection is capable, collects data in exponential
|
|
Nested PCR
|
increase the specificity of PCR with primer set 1&2 and primer set 3&4
|
