The chemical solution used in this experiment is the 1% coffee solution and the 2% coffee solution. The function of this coffee solution is to determine the differences in the heart rate (heartbeat) of the zebrafish. This is because the caffeine contained in the coffee can cause the significant change of the …show more content…
There is a declined reading of the larva heartbeat in the 1% coffee solution from the normal embryo solution that previously conducted. This abnormal observation of the larva heart rate may cause by the presence of the caffeine contamination in the environment of the larva fish. So, the physiological process of the larva zebrafish already being affected by the caffeine. Negrete (n.d.) stated that zebrafish that is exposed to the coffee solution which contained the caffeine cause the deformities and abnormalities of the embryo itself. This decreased in heart rate also contributed by the sensitivity of the embryo of the fish itself as it can easily being affected with the presence of the caffeine (Negrete,