When the pH increases the concentration decrease and vise versa. 10.Describe the differences in Acids and…
Introduction Many biological processes are pH dependent, to illustrate, stomach is at a relatively lower pH than other organs for proper function of digestive enzymes. In particular, buffer is essential for maintaining cellular functions. For instance, protein intake and metabolism generates about 70mM of H+ daily (Silverthorn, Johnson, Ober, Garrison, & Silverthorn, 2012), which could lower pH of human blood to as low as 3. To limit the drastic change in blood pH, human body has three buffer systems – bicarbonate, monohydrogen phosphate, and ammonia. Buffer is made up of weak acid and its conjugate base.…
State the optimum pH for sucrase activity and describe how sucrase activity changes at more acidic and more alkaline pH values. Table 2: Effect of Temperature on Sucrase Activity Optical Density 10 °CC (50 °F) 20 °C (68 °F) 30 °C (86 °F) 40 °C (104 °F) 50 °C (122 °F) 60 °C (140 °F) 70 °C (158 °F) 1 0.006 0.273 0.791 0.940 0.927 0.807 0.613 2 0.010 0.285 0.761 0.954 0.934 0.846 0.604 3 0.009 0.255 0.773 0.941 0.907 0.845 0.642 average 0.008 0.271 0.775 0.945 0.923 0.833 0.620 Effect of Temperature on Sucrase Activity 2. Was the rate of increase of sucrase activity higher at a pH of 8.5 or a pH of 5.5?…
Aim: This is an investigation to establish the effect of different temperatures (from 0°C -100°C) on the production of oxygen gas in a potato catalase reaction, using hydrogen peroxide as a substrate. The oxygen gas produced will be measured using a gas pressure sensor. Introduction: Enzymes are proteins, known as biological catalysts that increase the rate of reaction in a biochemical reaction without being chemically altered.…
The Effects of Substrate Concentration and Temperature on a Catalase Reaction Rate Introduction Catalase is a vital component in the human body. The most crucial of which would be Enzymes, these are protein catalysts. A catalyst speeds up a chemical reaction rate. Virtually all chemical reactions within the human body require a catalase enzyme to be completed in the proper amount of time.…
1.)During the lab and based on recorded observations it can be deduced that the enzyme catalase found in potatoes only reacted to hydrogen peroxide. This is do to the fact that before 10 ml of hydrogen peroxide was added to each test tube residing 3 potato samples and the designated liquid, no reaction was observed. Thus demonstrating the catalase's inability to react to vinegar, soda, water, detergent, and Na OH. Furthermore, shortly after the hydrogen peroxide was added to the liquids of diverse ph levels, a rapid and prominent throng of bubbles began to appear, which demonstrates the mediate reaction the hydrogen peroxide had on catalase.…
Studying the reaction of the enzyme catalase in different temperatures The purpose of this experiment was to measure the speed and the effectiveness of the enzyme catalase. The enzyme catalase purpose is to break down harmful substances and turn them into oxygen and water. The substance in this environment was hydrogen peroxide, and the study was to see how well the chicken liver would work if it was in a boiled environment, room temperature environment, an acid immersed environment and a basic environment.…
Catalase Enzyme Lab Write-Up Our goal with this lab is to discover how enzymes affect the production and destruction of harmful chemicals our body makes into harmless chemicals. Our body contains hundreds of enzymes in our cells and this lab will show us how they interact with parts and materials of our bodies. The enzyme we will be focusing on is called catalase. It’s job for the body is to break down hydrogen peroxide a harmful substance into two harmless substances oxygen and water.…
In this lab, we conducted a titration of ammonia and vinegar. We gradually added vinegar to a solution of water, ammonia, and our indicator (red cabbage water). The cabbage water is a visible sign of a solution ph level. ph is a measurement of how acidic or alkaline a solution is. A substance with a ph than 7 is a acid, and a substance with ph greater than 7 is a base.…
The second test tube 11 was identified as a gram negative bacteria. The biochemical tests that I performed that were positive were catalase, glucose, SIM, indole, citrate, and urea. First, the catalase test has the presence of an enzyme in the test isolated detected using hydrogen peroxide. Bacteria possess catalase with a small number of bacteria; isolation is added to hydrogen peroxide when bubbles occur. When the bubbles occurred on gram positive bacterium it was a conformation of being positive.…
Some enzymes can work at different pH levels. Two types of enzymes that operate at pH levels different than peroxidase are invertase and pepsin. Invertase works best at the pH level of 4.5 and pepsin can work at a pH of 1.5-1.6 (Effects of pH 2016). Both of these enzymes have optimal pH levels lower than the pH level of peroxidase. One problem with this experiment is the time between mixing the enzyme and placing it in the spectrometer may produce inaccurate results.…
During the first lab period, The gram stain show that the unknown bacteria was a gram neative rod shaped microbe. After this step was established, this next part was to select the best tests and mediums to determine the unknown bacteria. This medium are use to test the metabolic characteristics of the unknown bacteria. In order to identify the unknown bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For Metabolic tests, only Catalase test were performed on the unknown bacteria.…
Introduction: Enzymes have a huge effect on the human body and other organisms. Enzymes are catalysts that are used to accelerate the reaction process by lowering the activation energy. For this experiment the catechol oxidase was extracted from potatoes. The enzyme is called catechol oxidase because the oxygen is reacting to catechol. Catechol oxidase causes the browning of the fruits and vegetables because the compound is toxic to the bacteria.…
3.0 Hypothesis I assume that as the solution is treated with higher amount of fertilizer, the Spirulina cells will reproduce better and survive for longer time as there will be higher amount of nutrients supplied. Therefore, it is assumed that the Spirulina-concentration of the trials treated with 1.0mL fertilizer will be higher than those treated with 0.5mL or 0.0mL. Although wastewaters contain elements which are vital for the Spirulina growth, as it contain other unknown substances as well, its influence on the rate of growth of the Spirulina's cannot be unambiguously hypothesized. Since bicarbonate and sodium carbonate were not added to the effluent water but only to the portable water, it is assumed that the higher concentration of the…
Why? The catalase enzyme works best at pH 7 but it can perform well between 6.8 to 7.5. A 0.1 M Vinegar has a pH approximately 3. 2cm of them should have reduced the pH from 7 interfering with the breakdown of hydrogen peroxide.…