Gram Negative Unknown Lab Report #20 BIOL 3444-008 Giang Nguyen TA: Malini Kotak 11/05/2014 Abstract The experiment is conducted in order to identify a randomly given unknown gram-negative microorganism. By comparative analysis of biochemical tests that were performed throughout the semester to find out its characteristics and determine the identification of bacterium out of four potential unknown was given. To figure out the characteristics, T-streak was performed to isolate the sample from broth to a Tryptic Soy Agar plate, along with an additional slant in case something wrong with the original plate that can possibly affect the result.…
Bacteria is a type of Prokaryotes and RESEARCH Some Bacteria can be extremely harmful to many animals bodies including humans, these germs attack the immune systems and cause sickness and diseases, these illnesses occasionally result in death. Although not all bacteria are…
According to research by Bode Science Center this bacteria is a rod shaped but comes in many different types of bacilli and may trigger local infections such as peritonitis, meningitis, appendicitis, and a bloodstream infection (Rauhut, n.d.). This bacteria is Catalase, oxidase, decarboxylase positive. It does not ferment any sugars nor sulfur. It is motile but also can be no motile. This bacteria is a strict aerobic bacteria that was isolated from fecal matter and then later discovered it is also found in stale beer.…
Investigating Enterococcus faecalis’s structures and resistances to antibiotics Introduction Bacteria’s phenotype is commonly described as gram-negative or gram-positive from conducting a gram stain. A bacterium that is gram-negative tends to have cells composed of a thin peptidoglycan layer, and a gram stain of gram-negative bacteria are seen as red or pink through a microscope (Smith and Hussey, 2005). On the other hand, a bacterium that is gram-positive will have cells made of a thick peptidoglycan layer, and the gram stain of a gram negative bacteria would be seen blue or purple (Smith and Hussey, 2005). In particular, Enterococcus faecalis is a gram-positive bacterium which has been found in many infections in the urinary tract, surgical,…
There are many organisms in the world. In microbiology lab, I was given an unknown organism and I was told I must figure out what organism it truly is. I had the honor to conduct a few experiments to come to an conclusion on what the type and name of the organism was. Each member of my class was given a plate of agar and a random number that correspond to the plate of agar. I was given the number 191.…
The outer membrane is surrounded by special phospholipids composed of fatty acids called lipopolysaccharides that are attached to a glucosamine…
Gram + Cocci To identify the Gram + cocci I first performed a catalyst test which produced oxygen bubbles and indicated a positive catalase result. The next test I preformed was a mannitol salt test using a mannitol-salt agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test using a peptone-iron agar deep.…
100 Days of Horror with Bordetella Pertussis by Isabella Theamaree Fortun Bordetella pertussis is a fimbriated Gram-negative, pleomorphic, aerobic coccobacillus. It also has a surface associated microcapsule however the role of this is unknown. Bordetella species, in general, grow best at 35° to 37° C. 1 Their growth is fastidious, and this degree of fastidiousness is inversely proportional to its rate of growth.1 Among all Bordetella species, Bordetella pertussis is the most fastidious and slowest growing; this slow growth is caused by the growth inhibition caused by fatty acids, metal ions, sulfides, and peroxides.1 Furthermore, this bacteria affect humans only and it has no known animal reservoir.1 It is an extremely infectious organism that is transmitted via respiratory droplets from an infected person.3…
This section will be a narrative summary of the characteristic of my unknown bacterium that’ll touch base on the morphology, cell well structure, temperature tolerance, antibiotic resistance, physiology and other interesting experiment results. First is going through the morphology of my unknown bacterium; will be talking about the shape, cell wall structure, and other characteristic using the results from tests such as gram staining, simple staining, and spore staining. According to my lab notebook, the result from gram staining of my known is seen to be a rounded shape, cocci shape all bunched and clustered together like a bunch of grapes at 100x using the microscope (Kaddo, 2017, pg. 22). The color after the safranin wash from gram staining…
1. Non-pathogen name: Bacillus subtilis 2. Description of non-pathogen: B. subtilis is a mesophilic, gram-positive bacillus that is commonly found in soil, although it can also survive in plants (1). B. subtilis are facultative aerobes, using butanediol fermentation or nitrated ammonification when oxygen is unavailable (1). The bacillus is motile by using a single flagellum and commonly forms biofilms which contain several B. subtilis all traveling in the same direction (1).…
Literature Research: Staphylococcus is a type of Gram-positive bacteria that are commonly found together in random clusters. They are sometimes found in the same habitat as Micrococcus and can be differentiated because of the occurrence of cytochromes (5). They are typically facultative anaerobes or microaerophils and are able to produce catalase, making it possible for them to convert hydrogen peroxide to water to avoid potential damage. The major product of fermentation is lactate and they are not able to ferment citrate (4). There are several different species in the genus Staphylococcus such as Staphylococcus epidermidis and Staphylococcus aureus.…
Salmonella In our country, our government is very strict on the process of food being checked and being clean. At 1906 the Pure Food and Drug Act would be passed by president Franklin D. Roosevelt. This purpose was to make sure the the public would be safe from the many types of food they consume. Meat Inspection Act in 1906 followed by the Pure Food and Drug Act would also be passed for the same reason as the Pure Food and Drug Act.…
The Black Death, the bubonic plague, those are two names for the same disaster, one of the worst in recorded history, killing between 1/3 and 1/2 of the population of England, not to mention all the people killed in Asia and Africa. The name bubonic plague is a misconception, there were actually three forms of plague: the Bubonic, the Pnuemonic, and the Septicemic plagues, and they were all caused by the same germ, Yersinia Pestis. Yersinia Pestis, once called Pasteurella pestis1, "is a Gram-negative, rod-shaped coccobacillus, a facultative anaerobic organism"1. A Gram negative baterium can't be stained by crystal violet stain. This kind of bacterium has an extra, outer layer, that is made of proteins, that keeps the stain out9.…
The inside of the eukaryotic cell is a highly structured and organized, consisting of eukaryotic cells which externally may be bare or have cell walls, flagella, or cilia. In addition to that, they have internal…
Some of these organelles include the nucleus, which holds the cells DNA, mitochondria, which produce ATP from glucose and oxygen, known as cellular respiration, and the golgi body which packages and processes different substances leaving the cell. Prokaryotic cells are the simpler of the two cells and are single celled organisms, such as bacteria, that don’t contain organelles.…