The mucus layer microbiome is a part of the fish immune system, it is the first line of defense in protecting the fish from harmful external pathogens. The mucosal layer of the fish microbiome is examined to gain insight into the structure of the fish skin mucosa. In this experiment, an unknown strain of bacteria was isolated from the mucus layer of a Gambusia affinis, and then thoroughly tested to identify the FBI. The mucosa microbiome of the Gambius affinis is home to thousands of bacteria, however, by means of serial dilutions, the solution was made less concentrated to the point where a single cultured sample could be able to isolate one single bacteria. Then FBI 926 was isolated and further microbial identification test was used to paint
