Preliminary qualitative chemical tests show the presence of alkaloids, carbohydrates, saponins, steroids, terpenoids, phenolics, coumarins and fixed oil, etc.18
Desorption electrospray ionization was employed for fast and direct ambient detection of the anti-tumor drug, camptothecin, and its derivative, 9-methoxycamptothecin in Nothapodytes nimmoniana J. Graham. Different parts of the plant such as leaves, stems and bark were examined. The ion intensities suggest that the concentration in bark is higher than that in the leaves and stems7.
Nothapodytes nimmoniana J. Graham. Is a rich source of the potent alkaloid Camptothecin (CPT), 9-methoxy camptothecin …show more content…
Graham. such as dry leaf, fresh barks of plant, induced callus, somatic embryo and suspension culture (In exogenous medium and in pallet) in 100 mg/ml were analyzed for CPT content by TLC (Thin Layer Chromatography) and HPLC (High Performance Liquid Chromatography). TLC and HPLC analysis confirm the presence of CPT in dry leaf, fresh barks of the plant, induced callus and somatic embryo. Out of which bark extracts possessed a maximum amount of CPT, i.e. 377.77 μg/g, which supposed to be the first choice of CPT source. Results conclude that the CPT is present in all samples used except exogenous suspension culture, out of which soxhlet bark extract possesses the maximum amount of CPT24.
The variation in content of camptothecin from the bark of Nothapodytes nimmoniana J. Graham., using HPLC analysis. The bark drug receives three seasons viz. monsoon, winter and summer in months of August, December and May. This serves a rationale to our study, where we find monsoon (August) to accumulate higher levels of CPT (1.337 gram / 100 gram dry bark powder) in barks of Nothapodytes nimmoniana J. Graham. As compared to summer …show more content…
In our investigation, the CPT content was highest in roots followed by fruits then in stems and leaves of Nothapodytes foetida (Wight) Sleumer. The similar pattern variation in accumulation of CPT is observed. The results of the present study also showed that concentration of CPT accumulated in the root of Nothapodytes foetida (Wight) Sleumer collected in the month of February was about 13 fold higher in comparison with the roots when collected in October26.
Leaf and stem materials from ten individuals of Nothapodytes nimmoniana J. Graham. From each locality were collected. Then simple micro extraction method done with methanol. The Stem were found to be better options over leaf materials for CPT accumulation of Nothapodytes nimmoniana J. Graham. along the Western Ghats,
Desorption electrospray ionization was employed for fast and direct ambient detection of the anti-tumor drug, camptothecin, and its derivative, 9-methoxycamptothecin in Nothapodytes nimmoniana J. Graham. Different parts of the plant such as leaves, stems and bark were examined. The ion intensities suggest that the concentration in bark is higher than that in the leaves and stems7.
Nothapodytes nimmoniana J. Graham. Is a rich source of the potent alkaloid Camptothecin (CPT), 9-methoxy camptothecin …show more content…
Graham. such as dry leaf, fresh barks of plant, induced callus, somatic embryo and suspension culture (In exogenous medium and in pallet) in 100 mg/ml were analyzed for CPT content by TLC (Thin Layer Chromatography) and HPLC (High Performance Liquid Chromatography). TLC and HPLC analysis confirm the presence of CPT in dry leaf, fresh barks of the plant, induced callus and somatic embryo. Out of which bark extracts possessed a maximum amount of CPT, i.e. 377.77 μg/g, which supposed to be the first choice of CPT source. Results conclude that the CPT is present in all samples used except exogenous suspension culture, out of which soxhlet bark extract possesses the maximum amount of CPT24.
The variation in content of camptothecin from the bark of Nothapodytes nimmoniana J. Graham., using HPLC analysis. The bark drug receives three seasons viz. monsoon, winter and summer in months of August, December and May. This serves a rationale to our study, where we find monsoon (August) to accumulate higher levels of CPT (1.337 gram / 100 gram dry bark powder) in barks of Nothapodytes nimmoniana J. Graham. As compared to summer …show more content…
In our investigation, the CPT content was highest in roots followed by fruits then in stems and leaves of Nothapodytes foetida (Wight) Sleumer. The similar pattern variation in accumulation of CPT is observed. The results of the present study also showed that concentration of CPT accumulated in the root of Nothapodytes foetida (Wight) Sleumer collected in the month of February was about 13 fold higher in comparison with the roots when collected in October26.
Leaf and stem materials from ten individuals of Nothapodytes nimmoniana J. Graham. From each locality were collected. Then simple micro extraction method done with methanol. The Stem were found to be better options over leaf materials for CPT accumulation of Nothapodytes nimmoniana J. Graham. along the Western Ghats,