Paper Chromatography
Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted:
Materials
Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre)
Paper Clips/Sellotape & Skewer Stick
Water soluble black marker
Water
Procedure:
Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the papers onto a skewer stick, ready for use.
Place water (acts as solvent) into the beaker making sure there is enough so that the spot on the papers can at least be partly submerged when placed in.
Place the paper on the skewer stick into the beaker so that the skewer limits the paper from being completely …show more content…
Testing of antibiotics and determining the pollutants in water: Done to verify purity of water and to test antibiotics to see if it is capable of doing its job.
Used in DNA and RNA fingerprinting: Done in pathological laboratories to detect the presence of alcohol or chemicals in blood and also for crime scene investigations (forensic evidence). Used in the separation of amino acids, carbohydrates, fats, vitamins, and more: Done for extensive research and even medical purposes.
Thin Layer Chromatography (TLC)
Thin Layer Chromatography is a chromatographic technique used to separate the components of a mixture using a thin stationary phase substance. It is operated similarly to paper chromatography.
Materials
Silica Plate, Tweezers & Gloves, Pencil & Ruler
Unknown Dye (X), Unknown Dye (Y) & Unknown Dye (X & Y)
3 Pasteur Pipettes & UV Light (Ultra-Violet)
Jar or Beaker with Lid
Solvent (Ethyl Acetate-Hexane [1:1] is most
Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted:
Materials
Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre)
Paper Clips/Sellotape & Skewer Stick
Water soluble black marker
Water
Procedure:
Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the papers onto a skewer stick, ready for use.
Place water (acts as solvent) into the beaker making sure there is enough so that the spot on the papers can at least be partly submerged when placed in.
Place the paper on the skewer stick into the beaker so that the skewer limits the paper from being completely …show more content…
Testing of antibiotics and determining the pollutants in water: Done to verify purity of water and to test antibiotics to see if it is capable of doing its job.
Used in DNA and RNA fingerprinting: Done in pathological laboratories to detect the presence of alcohol or chemicals in blood and also for crime scene investigations (forensic evidence). Used in the separation of amino acids, carbohydrates, fats, vitamins, and more: Done for extensive research and even medical purposes.
Thin Layer Chromatography (TLC)
Thin Layer Chromatography is a chromatographic technique used to separate the components of a mixture using a thin stationary phase substance. It is operated similarly to paper chromatography.
Materials
Silica Plate, Tweezers & Gloves, Pencil & Ruler
Unknown Dye (X), Unknown Dye (Y) & Unknown Dye (X & Y)
3 Pasteur Pipettes & UV Light (Ultra-Violet)
Jar or Beaker with Lid
Solvent (Ethyl Acetate-Hexane [1:1] is most