Catalase

Gram Stain, Oxidase Test, Catalase Test, and EnteroPluri-Test . Highlighted in the experimental flow chart it states the tests performed and the results that led to the identification of isolate A and isolate B in sample 4-24. Each test…

that match up with the enzymes to cause the reactions. An example of an enzyme is catalase (the enzyme used in this lab); catalase helps break down hydrogen peroxide(2H2O2 ---- O2 + 2H2O). Why? Hydrogen peroxide is one of the most toxic chemicals. But we put it on our cuts to clean and heal…

bacteria only that are able to ferment lactose. Also, mannitol agar was used which isolates and detects gram-positive bacteria. As well as blood agar, which is a nutritive media with differential properties in respect to hemolysis. The catalase test indicated catalase production and the coagulase test indicated a lack clotting in the tube. These tests were used for…

We first conducted the catalase test. We collected a microscope slide and placed it inside of an empty petri dish, attempting to rule out contamination. We then scoped a sample of our unknown bacteria from the LB agar (Luria Broth), then added a drop of 3% H202 (Hydrogen Peroxide). We observed the slide for a reaction to take place. If a reaction was observed, we knew that the species of our unknown bacteria was able to produce catalase to negate the effects of H202. For the…

In this part of the lab, we pour 20 mL of Hydrogen Peroxide and 1 mL of yeast catalase in the reaction chamber and then submerge it in the water bath. After it was done, it can be concluded that the gas levels increased because when we first started timing it, the gas levels were at 76, but as time went by, the gas levels started to increase and in the end of the last interval, the gas level increased all the way up to 100. This result occurred because there were many active sites that were…

Trend The constant, 0 v/v % hydrogen peroxide solution, did not react with the catalase, therefore it was recorded as 300+ seconds and had a reaction rate of 0 seconds-1. The 0.1 v/v % hydrogen peroxide concentration recorded a reaction rate of 0.17 seconds-1. The 0.2 v/v % hydrogen peroxide concentration, recorded a lower reaction rate than the 0.1 v/v% at 0.14 seconds-1. The 1 v/v % hydrogen peroxide concentration recorded higher at 0.49 seconds-1. The 3 v/v % hydrogen peroxide concentration…

releases its products”(Boundless, 2015). This lab demonstrates an enzyme’s functionalities by using the enzyme called catalase. Catalase helps break down hydrogen peroxide, the substrate, to water and oxygen, the products. To illustrate its effectiveness “Catalase prevents the accumulation of and protects cellular organelles and tissues from damage by peroxide... In mammals, catalase is found…

Liver Lab By: Jonah Stillman Background Information: Catalase enzymes are inside of nearly every living organism that is exposed to oxygen. Some examples of catalase enzymes are bacteria, plants, and animals. The main job of a catalase enzyme is to catalyze the decomposition of hydrogen peroxide to water and oxygen. When an enzyme is exposed to hydrogen peroxide or certain other chemicals it is prone to react. Catalase enzyme reactions also depend on the temperature they are exposed to. As…

biomolecules in the cells that would eventually result in cell damage or cell death. Catalase plays an important role in regulating hydrogen peroxide in plants because catalase activity affects the concentration of H2O2. Catalase is an antioxidant enzyme made up of polypeptides and iron. Materials: Water bin filled ¾ of the way full (distilled) 3 Graduated cylinders: 10 mL (2), 25 mL 5 containers of potato catalase with different temperatures Reaction…

decrease. Our data shows that catalase is very active near 37℃, which is the average bodily temperature of many mammals (humans, cattle, pigs, etc.). However, the catalase activity is not the most active at this point. In the class data, it says that at the hottest level it was an average of 7.28 seconds, while at 40℃ (closest to 37℃) it was 15.85 seconds. While the reaction is still quick, it has room to grow. Catalase temperature specificity must say that a catalase will be at its bottommost…