Endospore

This section will be a narrative summary of the characteristic of my unknown bacterium that’ll touch base on the morphology, cell well structure, temperature tolerance, antibiotic resistance, physiology and other interesting experiment results. First is going through the morphology of my unknown bacterium; will be talking about the shape, cell wall structure, and other characteristic using the results from tests such as gram staining, simple staining, and spore staining. According to my lab…

CHAPTER THREE MATERIAL AND METHODS 1. PHYSICO-CHEMICAL CHARACTERCITICS: The effluent collected were analyzed in the laboratory for pH, acidity, alkalinity, salinity, total dissolved solid(TDS), conductivity, total hardness, calcium hardness, magnesium hardness, total solid, total suspension solid, turbidity, BOD, DO and COD using standard methods (APHA,2005). A.DETERMINATION OF pH: The electrode of pH meter was calibrated with standard buffer solutions of pH 4, 7 and 9. The electrode was then…

Following Gram staining and microscopic examination, Unknown 15 was identified to be a Gram positive coccoid. Based on these results, all of the Gram negative genera were eliminated including: Enterobacter, Citrobacter, Klebsiella, Escherichia, Pseudomonas, Serratia, Alcaligenes, Neisseria, Proteus, Salmonella, Shigella, Erwinia, Veillonella, and Flavobacterium (5). Bacillus, Lactobacillus, Listeria, Kurthia, and Arthrobacter were eliminated because they did not match the coccoid shape of…

The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if…

morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and oxidase test were negative. The FTM, catalase, MSA and blood agar tests were positive. After the Acid Fast stain, the cells were blue and non-acid fast. After the Endospore stain, the cells were pink. This indicated that they were non-endospores. DISCUSSION The purpose of this experiment was to search through soil, isolate and characterize new…

positive because of purple dye seen under the microscope. Microbe B was a gram-negative because of the light pink dye seen under the microscope. The gram stain tests can be seen in figure 1. The endospore stain was used to verify that microbe C contained endospores. When tested, microbe C did have endospores. Table 4 describes selective media tests. EMB was used for lactose fermentation and growth of gram-negative bacteria . Microbe A and microbe B turned dark purple and were able to produce…

performed were chosen based on the identification table that was given from the lab manual based on gram staining. The first test I performed was to check for endospore forming, my results came back positive the first time. However, it was difficult to verify if it was positive or not which had me test three more times resulting in non endospore forming.…

Isolated Colonies and Soil Samples A. Purification of Serratia marcescens and Staphylococcus epidermis The objective was to grow isolated colonies on a nutrient agar medium (NA). Many microorganisms found in soil are able to grow on this medium. Since there are many organisms that are found in the soil, only a few of this organisms are adapted to live in this specific medium obtained. The NA plate is the first way to isolate microorganisms because the organisms that will be left on the plate…

Procedures in clinical laboratories involve working with biological samples that may contain viruses such as HIV and/or Hepatitis B and other medically important bacteria. Therefore, it is important that the laboratory workers protect themselves by using the correct personnel protective equipment (PPE) and by so doing protect the members of the public. In the microbiology laboratory, sterile techniques must always be used to prevent cross contamination of specimens but also to prevent…

isolation process was performed by streaking a mixed culture onto a nutrient agar plate, then transferring the isolated cultures after incubation for biochemical testing. S. ureae is discussed below. Biography S. ureae is a coccus, gram-positive, endospore-forming, chemoorganotrophic, obligate aerobe and has motility via flagella. (Sporosarcine, 2006). It is typically found in soils at the bases of trees where dogs have urinated, and has also been found in manure (Dworkin, Falkow, Rosenberg,…