different places. Also, different types of bacteria grow in different conditions. From prior experience, I remembered that to grow bacteria in a petri dish one must follow the proper technique. I chose to test how much bacteria grows on toilet handle in the bathroom. My prediction for the experiment before it was conducted was that there would be a large amount of bacterial growth on the handle of a public bathroom toilet. This was my hypothesis because many people touch the toilet handle after using the bathroom and before washing their hands. I predicted that the most bacteria would be found on objects that were touched by many different people throughout a day. To conduct this experiment, everyone in the class got in pairs. Each pair shared a petri dish to grow bacteria in. The first thing the partners did once they got their petri dish was to turn the dish upside…
most growth compared to the other strain of yeast. Methods In this experiment, we used two different sugars, sucrose and glycerol, and individually placed them into two different strains of yeast. In this experiment each of the petri dishes contained 1/3 of 143 flo ↑, 140 flo ↓, and 138 WT. However, 138 WT was not inoculated because it was not being tested. For this experiment, we inoculated two kinds of sugars on a sterile cotton swab and then swabbed two petri dishes with sucrose and two…
Table of Contents: Introduction………………………………………………………………………………………………………………………..2 Aim……………………………………………………………………………………………………………………………………….2 Background Info……………………………………………………………………………………………………………………2 Hypothesis…………………………………………………………………………………………………………………………….2 Variables……………………………………………………………………………………………………………………………….2/3 • Independent…………………………………………………………………………………………………………………………..2 • Dependent……………………………………………………………………………………………………………………………..3 •…
Method: Purpose: To investigate how Porcellio Scaber reacts in terms of orthokinesis to different humidities in their habitat. Equipment- 75 slaters which range from 5-10mm in length (kept in a plastic container with holes in the lid, with dirt and bark so that they are kept in a natural environment as possible so can react naturally during experiment.) 5x petri dish set ups labelled from 50-90% (One petri dish on the bottom will contain glycerol and have a mesh/gauze hot glued over top…
Methods: To start this experiment place four seeds in four separate petri dishes with a damp paper towel. Once the seed germinates and the plant has begun to sprout, move the seeds to four separate pots, and add soil. Once the plants have begun to sprout take the measurement of each plant and record your data for day zero. Next, take three glass containers of greatly varying volumes (41,4185cm3, 2,250cm3, and 602cm3 were used). Before the plants are placed underneath these containers, give…
colony will be transferred to a clean glass slide and will be smeared and heat-fixed. The slide will be flooded with crystal violet for 1 minute and rinsing with distilled water. Next, the slide will be flooded with iodine solution for 1 minute, and rinsed with distilled water. It is then decolorized with alcohol and will be drained, and counterstained with safranin for 30 seconds. The slide will be rinsed and excess stain will be wiped out, and then air dry. Finally, the slide will be observed…
To change solid agar into liquid, get 4 Petri dishes out and ready for use. Next, agar needs to be a boil, either on heating pot or stove, not in a microwave. Agar bottle needs to be placed inside the pot, with water in it to be a boil. Water level needs to be right under the label on the agar bottle, with the cover losing, or it will explode. Agar needs to be boil for about 30 to 45 minutes, until agar liquefied. Then pour agar inside the petri dish, about half full. Make sure agar is fully…
Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…
Objective I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient…
loop? a. After sterilizing the loop over an open flame, depending if the bacteria is in a liquid or solid form, we dip the loop inside the sample test tube and dip the inoculated loop straight down in the tube. If the sample is a solid the sterilized loop should barely touch the culture. 4. Why do we have to wait for the loop to cool before collecting a sample? a. We have to wait for the loop to cool before collecting samples because we may kill the bacteria or melt the agar jelly that is…