Culture vessels were prepared by following the instructions in the Carolina Drosophila Manual. One-part Drosophila medium was deposited into the bottom of the culture vessels with one-part water. Then the medium was left to firm. Once the medium was firm, the yeast was added as a food source to sustain development, medium is for the larva to eat, and yeast is for adult flies to eat. A piece of plastic mesh was added to the culture vessel to give the fruit flies more surface area, which was needed so that they would not crush one another or get stuck in the medium. To sex the fruit flies, the flies were first transferred to an empty culture vessel to be anesthetized with Fly-Nap. Then the flies were moved to a wide field stereomicroscope and separated by gender. Male fruit flies have heavy dark bristles around the base of the genitalia, and females do not. After the F1 flies were sexed, males and females were put into a culture vessel to breed the F2 generation. As the F2 flies hatched over a seven-day period they were counted and categorized. The flies were then categorized by traits and gender (Flagg, R. O.,
Culture vessels were prepared by following the instructions in the Carolina Drosophila Manual. One-part Drosophila medium was deposited into the bottom of the culture vessels with one-part water. Then the medium was left to firm. Once the medium was firm, the yeast was added as a food source to sustain development, medium is for the larva to eat, and yeast is for adult flies to eat. A piece of plastic mesh was added to the culture vessel to give the fruit flies more surface area, which was needed so that they would not crush one another or get stuck in the medium. To sex the fruit flies, the flies were first transferred to an empty culture vessel to be anesthetized with Fly-Nap. Then the flies were moved to a wide field stereomicroscope and separated by gender. Male fruit flies have heavy dark bristles around the base of the genitalia, and females do not. After the F1 flies were sexed, males and females were put into a culture vessel to breed the F2 generation. As the F2 flies hatched over a seven-day period they were counted and categorized. The flies were then categorized by traits and gender (Flagg, R. O.,