A group of three proteins known as the ‘prepriming complex’ are responsible for unwinding the double helix and keeping it separated. The first step is carried out by the DnaA protein. It binds to the origin nucleotides resulting in an AT rich region. The AT rich region sparks the separation of the double strand into single strands of DNA, the ATP dependent process of melting. DNA helicases are responsible for unwinding the helical structure of DNA. They bind to the single stranded DNA created by DnaA protein and move toward the double stranded DNA, turning it into single strand by forcing the strands apart. DnaB helicase is the primary helicase in action in E.Coli. The last part of the prepriming complex is single stranded DNA-binding proteins (SSB). Their job is to create the single stranded template required for the actions of polymerases and to protect the DNA from degradation by nucleases. SSB proteins bind to the single stranded DNA made by the helicases and keep them
A group of three proteins known as the ‘prepriming complex’ are responsible for unwinding the double helix and keeping it separated. The first step is carried out by the DnaA protein. It binds to the origin nucleotides resulting in an AT rich region. The AT rich region sparks the separation of the double strand into single strands of DNA, the ATP dependent process of melting. DNA helicases are responsible for unwinding the helical structure of DNA. They bind to the single stranded DNA created by DnaA protein and move toward the double stranded DNA, turning it into single strand by forcing the strands apart. DnaB helicase is the primary helicase in action in E.Coli. The last part of the prepriming complex is single stranded DNA-binding proteins (SSB). Their job is to create the single stranded template required for the actions of polymerases and to protect the DNA from degradation by nucleases. SSB proteins bind to the single stranded DNA made by the helicases and keep them