Kanamycin was co-spray-dried with rifampicin using a Buchi Mini Spray-dryer. The surface composition was determined by X-ray …show more content…
XPS and ToF-SIMS showed the surface of the combination powder was enriched with rifampicin. Aerosolization was dramatically improved with the fine particle fraction (FPF) increasing from 29.5 ± 0.2% (kanamycin-only) to 78.2 ± 1.3% (kanamycin/rifampicin combination). All the spray-dried powders were noncrystalline. The combination powder was stable at 15% and 53% RH and 25 ± 2°C during one-month storage in an open Petri dish and non-toxic (max concentration tested 50 µg/mL) to human alveolar and bronchial cell-lines.
Surface enrichment of kanamycin by hydrophobic rifampicin improves aerosolization. Improved aerosolization may help to combat drug-resistant local infections by facilitating high dose delivery to deep lung. Further studies are required to distinguish the potential mechanisms for improved aerosolization and test the long-term