The human microbiome is the cumulative genomes of all the different types of microbes ranging from bacteria, fungi, and viruses that live in and outside of the human body. We have many mutualistic, commensal, and even detrimental microbes living in our bodies. There are commensal microbes that sometimes are also responsible for the infection due to the microbe containing a pathogenic strain. In our laboratory, there appears to be 40 patients that are experiencing a visible skin infection on their face, and the infection does not seem to respond to the current antimicrobial treatment. Upon observation of this skin infection, it appears to be a rosacea infection. To confirm and verify the identity of the infection, …show more content…
In both techniques, we will first cleanse the infected area on the face before swabbing a culture to avoid collecting normal microbiota as we only want the infected agent using aseptic technique. In order to identify the pathogen, we will cultivate, isolate, and then identify the pathogen. In the classical method, we will culture the specimen in an agar to achieve more growth. We can then look at the morphology of the cell shape, size, and arrangement through light microscopy. Sometimes, an infection can be diagnosed solely by the patient’s signs and symptoms. We can also use Gram stain reaction and acid-fast reaction. Electron microscopy can also be used to identify whether the pathogen contains cell wall, flagella, or fimbriae. Then, we will use biochemical tests to examine the pathogen’s peptide composition of the cell wall, makeup of the membrane and lipids. A dichotomous key can allow us to organize and trace a route to eliminating various characteristics that the microbe does or does not contain to identify the correct microorganism. Utilizing the phenotypic method of classification, we can then identify the microbe responsible for the skin …show more content…
That infection was diagnosed to be rosacea based on the laboratory tests performed as well as examining the patient’s signs and symptoms of redness, small red visible bumps and spots on the facial skin. The enhanced expression of toll-like receptor 2 from a pathogenic strain in rosacea results in inflammation of the skin. In the human microbiome, we are made up of many microbes ranging from good, neutral, and bad ones. It was discovered that even a neutral microbe can cause harm because of a pathogenic strain. The normal strain plays a strong role in maintaining healthy skin. However, when there is a pathogenic strain, it can affect the skin tremendously. Harmful strains can induce harmless ones into becoming pathogenic through different portals of entry that are exposed, and in the skin, certain bacteria can also initiate infection. All in all, this outbreak that the patients acquired was diagnosed as a rosacea infection where the toll-like receptors 2 from a pathogenic strain of rosacea was the cause of antibiotic resistance. We used both the classical methodology and molecular methodology to identify the pathogen that caused the