It was then incubated at 37˚C for 48 hours. Afterwards the plate was observed for any color change and results were recorded (3). The Urease test was performed by heavily inoculating the broth, with a pure isolated bacteria colony from the gram negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 8 days. Afterwards the urea broth was observed for any color change and the result was recorded (3). The Methyl Red test was performed by inoculating the MR-VP broth, with a pure isolated bacteria colony from the gram negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 24 hours. After 24 hours 15 drops of the reagent, methyl red, was added to the MR-VP. After several minutes the results were observed and recorded (3). The Voges-Proskauer test was performed by inoculating the MR-VP broth, with a pure isolated bacteria colony from the gram-negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 3 days. After 3 days, 15 drops of the reagent barrits A, and 5 drops of the reagent barrits B was added to the MR-VP broth. After one hour, results were observed and recorded
It was then incubated at 37˚C for 48 hours. Afterwards the plate was observed for any color change and results were recorded (3). The Urease test was performed by heavily inoculating the broth, with a pure isolated bacteria colony from the gram negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 8 days. Afterwards the urea broth was observed for any color change and the result was recorded (3). The Methyl Red test was performed by inoculating the MR-VP broth, with a pure isolated bacteria colony from the gram negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 24 hours. After 24 hours 15 drops of the reagent, methyl red, was added to the MR-VP. After several minutes the results were observed and recorded (3). The Voges-Proskauer test was performed by inoculating the MR-VP broth, with a pure isolated bacteria colony from the gram-negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 3 days. After 3 days, 15 drops of the reagent barrits A, and 5 drops of the reagent barrits B was added to the MR-VP broth. After one hour, results were observed and recorded