When Murata-Hori used a negative mutant form of Aurora-B kinase, they noticed that the movement of chromosomes was abnormal (Murata-Hori & Wang, 2002). With the mutant form, the chromosomes was seen to be arranged into two elongated bundles on the sides of the spindle axis instead of forming a metaphase plate in the equatorial region of the cell (Murata-Hori & Wang, 2002). Since the mutant used was kinase-inactive, there were a number of other adverse effects seen. When this mutant was overexpressed, motor proteins such as dynein and CENP-E were not able to function (Murata-Hori & Want, 2002). Also, the kinetochores were unable to attach to the spindle arrays (Murata-Hori & Want, 2002). These effects were more adverse, which suggests that the phosphorylating activity of the Aurora-B kinase is very important for the proper segregation of chromosomes during
When Murata-Hori used a negative mutant form of Aurora-B kinase, they noticed that the movement of chromosomes was abnormal (Murata-Hori & Wang, 2002). With the mutant form, the chromosomes was seen to be arranged into two elongated bundles on the sides of the spindle axis instead of forming a metaphase plate in the equatorial region of the cell (Murata-Hori & Wang, 2002). Since the mutant used was kinase-inactive, there were a number of other adverse effects seen. When this mutant was overexpressed, motor proteins such as dynein and CENP-E were not able to function (Murata-Hori & Want, 2002). Also, the kinetochores were unable to attach to the spindle arrays (Murata-Hori & Want, 2002). These effects were more adverse, which suggests that the phosphorylating activity of the Aurora-B kinase is very important for the proper segregation of chromosomes during