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35 Cards in this Set
- Front
- Back
Immediately after birth we gain waht |
Internal and external bacterial flora that constantly fluctuating in number and species and remain with us throughout our lifetimes |
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What does the skin serve as |
An effective barrier against microbial invasion |
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What does the skin contain |
Resident populations |
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Are resident populations generally harmless |
Yes, they may even provide some protective capacity |
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Why do transient species appear? |
As a result of contact with the surroundings |
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Are trasnient species variable
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Yes, they are highly variable |
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Can transient species be washed off easily |
Yes |
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Where do resident species live |
In or on the skin
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What do the resident species use as nutrient sources |
Skin secretions and wastes
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What is the exercise trying to enumerate |
2 groups of bacterial flora Promionibacterium Micrococcaeae |
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What are the different types of skin using |
Dry, moist, oily |
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Can this procedure recorver 100% of the resident bacterial cells |
No Due to their inaccessibility |
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What does the donor do |
Bacterial flora will be sampled from them |
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Should donors currnetly be using a topical anti-bacterial product? |
No |
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Is there a simple relationship between personal hygiene and density and type of skin flora? |
No |
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The surface to be sampled is _____ |
Horizontal
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How long will each sample take |
2 minutes |
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How to do a wash |
Place a sterile sampling cylinder on the area to e sampled and apply only enough pressure to ensure no leakage of the wash fluid. Pipette 1 ml of wash fluid into the cylinder. Rub the skin for 1 minute with a sterile glass rod Endeavor to rub the entire area of skin enclosed by the cylinder Withdraw the wash fluid with a pipette and dispense into an empty sterile test tube |
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How many washes should you do of the same area |
Two and then pool the two wash samples |
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Why do we draw up the fluid with a 5 ml pipette
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To estimate the total volume |
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How much of the dilutions should we spread onto the plates |
0.1 ml |
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What are the two media we are using |
RCMF HBA |
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Is RCMF incubated anaerobically or aerbocially |
Anaerobically |
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What does RCMF select for |
Propionibacterium |
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HBA is what |
Heated blood agar |
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Is HBA incubated aerbocially or anaerobically |
Aerobically |
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What does HBA allow for the growth of |
Micrococcaceae |
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Where do we place the RCMF plates |
In an anerobic jar |
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How do we incubate HBA? |
Aerobically |
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What do we do when we first examine the HBA and RCMF plates |
count and note the different colony types
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What do we do after we count and note the different colony types |
Perform gram stains from represetnative colonies |
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Will we perform diagnostic tests for identification |
No |
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What are aerobic flora |
Gram positive cocci Often in clusters but not chains Colonies are white, gray, golden, yellow, hemolytic or non-hemolytic |
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What are anaerobic flora |
Gram-positive rod or club shaped cells Often clumped in groups resembling picket fences Colonies are brown-yellow, pink and mucoid or pink and dry with scalloped edge |
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When doing hte calculation what must we take into consideration |
Dilution used |