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30 Cards in this Set
- Front
- Back
Trypsin Tx. |
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Cyanogen Bromide Tx. |
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Chymotrypsin Tx. |
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Carboxypeptidase Tx. |
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Thermolysin Tx. |
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Pepsin Tx. |
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Endopeptidase Tx. |
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Elastase Tx. |
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2-Mercaptoethanol Tx. |
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4 Modes of Enzyme Regulation |
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4 types of Reversible Inhibition |
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Ki and Stability of Reversible Inhibitors |
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Enzyme Maturation |
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Ki and Stability of Irreversible Inhibitors |
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Allosteric Enzymes |
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Isoenzymes |
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Allosteric Effectors |
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Reversible Phosphorylation |
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Chymotrypsin Mechanism |
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Trypsin Tx. Of Chymotrypsinogen (target is Arg15) |
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Autolysis of (pi) Chymotrypsin |
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CM - Step 1 |
Substrate Binding |
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CM - Step 2 |
Nucleophilic Attack |
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CM - Step 3 |
Substrate Cleavage |
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CM - Step 4 |
Water Comes In |
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CM - Step 5 |
Water Attacks |
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CM - Step 6 |
Break-off from the Enzyme |
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CM - Step 7 |
Product Dissociates |
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Chymotrypsin - Catalytic Triad |
His 57 Asp 102 Ser 195 |
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Lysozyme - Enzymatic A.A. |
Glu 35 Asp 52 |