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18 Cards in this Set
- Front
- Back
V= d[P] /dt is also what?
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V= -d[S]/dt
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With decreasing [S] and increasing [P] , what wil be lost with time of reaction?
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linearity, in some cases P may inhibit E at higher concentrations.
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All E in the form of ES is what?
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Saturated
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When saturation is at high [S] increasing the [S] doesn't do what?
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increase the rate of reaction any further
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The velocity obtained at saturating [S] is what?
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the maximum velocity (Vmax)
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What is Michaelis's constant?
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[S] is 1/2 the Vmax. The term is called the Km
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What units are Km expressed in?
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moles/liter or mg/ml
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V=Vmax[S]/[S]+Km is also what?
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Km=[S](Vmax-V)/V
so when, 2V=Vmax, Km=[S] |
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Km is sometimes associated with what?
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the strength of binding of the substrate for the enzyme.
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A substance with low dissociation rate is what type of affinity?
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higher affinity
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A substrate with low dissociation rate (high affinity)for the enzyme would reach what?
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half-maximal velocity at lower [S]
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A turnover number of enzyme reactions is what?
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Kcat
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The larger the Kcat the more often what happens on the surface of the enzyme?
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catalytic events
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Units of Kcat are per what?
What does Kcat measure? |
per seconds, that is (s^-1)
measures number of substrate molecules turned over per enzyme per second. Values range from chymotypsin (0.1) to carbonic anhydrase (100,000). |
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The Kcat/Km determines the efficency of the enzyme-mediated reaction by giving what?
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The percentage of the effective collisions.
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True or False
Some enzymes approach the theoretical limit where close to every collision result in catalysis. |
True.
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Cells far from the thermodynamic equilibrium are in what state?
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The steady state.
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A dead cell is at what?
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equilibrium
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