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38 Cards in this Set
- Front
- Back
Types of Media |
Differential Selective General |
|
Differential |
media that may grow different types of bacteria. eg. macConkey, Eosin MTB |
|
Selective |
used to grow only desired bacteria and to inhibit the growth of unwanted bacteria. (e.g Mannitol Salt, Sabouraud-Dextrose) |
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General |
most bacteria will grow. eg. nutrient agar |
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Plates |
use petri dishes fill just enough to cover the bottom of the dish. 45 degrees |
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Deeps |
Approximately 10-20 ml of media is poured into a test tube and capped. |
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Slants |
same as deeps, but media is cooled at 45 degrees |
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Broth |
uses same ingredients except for agar. media stays in liquid form |
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Complex |
grows most heterotrophic organisms, composition varies from batch to batch |
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Defined |
grows specific heterotrophs and often mandatory for chemoautotrophs. exact composition of media |
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Selective media |
suppresses unwanted microbes |
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Differential |
Distinguishes colonies of specific microbes from others |
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Enrichment |
similar to selective but designed to increase # of desired microbes to a detectable level. |
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Reducing |
Reduces oxygen concentration. required for obligate anaerobes |
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Blood Agar |
rich, nonselective, differential 5% sheep blood to complex media other bacteria release hemolysins (enzymes that break down RBC |
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Beta-Hemolytic |
lyse RBC and degrade hemoglobin |
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Alpha-hemolytic |
lyse RBC, do not degrade hemoglobin |
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Gamma-hemolytic |
no harm to RBC |
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Eosin Methylene Blue(EMB) |
selective and differential media
comobo of dyes, eosin, and MTB only allow Gram (-) to grow |
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Gram (-) bacteria that ferment lactose |
produce acid, blue-black appearance |
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Gram (-) bacteria that ferment lactose rapidly |
very distinct green-metallic sheen |
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3) MacConkey Agar (MAC) |
differential plating medium selective for gram - detect and isolate enteric bacteria |
|
4) Mannitol Salt Agar(MSA) |
highly selective medium for staphylococci. 7.5% of NaCl |
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Pathogenic Staphylococci |
ferment mannitol>lower ph> red turns yellow |
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Non-pathogenic Staphylococci |
do not ferment mannitol>ph same> stays pink |
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Trypticase Soy Agar (TSA) |
work horse non-selective non-differential medium, has proteins and dextrose |
|
Sabouraud Dextrose Agar (SDA) |
selective, growth of fungi (yeast and molds) due to low ph |
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Phenylethanol Agar (PEA) |
selective for gram + phenylethanol is bacteriostatic agent good to grow staph or strept |
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Triple Sugar Iron Agar (TSI) |
differentiation of gram - enteric bacilli Dextrose, sucrose, and lactose (all 1%) iron source |
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top and bottom all yellow |
ferment all sugars |
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top red, bottom yellow |
only dextrose fermented |
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top and bottom red |
non-fermenter |
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hydrogen sulfide production |
black precipitate |
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Gas production |
looks like cracks |
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Brilliant Green Agar (BG) |
highly selective isolation of salmonella |
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E. coli |
lactose fermenter(yellow-green colonies) |
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Salmonella spp. |
non-lactose fermenter(red colonies) |
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Nutrient Agar(NA) |
work horse non-selective and non differential |