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12 Cards in this Set
- Front
- Back
What is the purpose of this staining methodology?
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To distinguish between Gram-Positive and Gram-Negative bacteria
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Name the most critical step in the gram staining that brings the difference in the final staining of G+ and G- bacteria?
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Decolorization
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The mordant of Gram staining is _________________
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The mordant of Gram staining is Iodine.
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The decolorizing agent of Gram staining is ________________
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The decolorizing agent of Gram staining is ethyl alcohol.
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The second stain of Gram staining is _____________
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The second stain of Gram staining is Safranin
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What is the difference between Gram -Positive and Gram-Negative?
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Gram positive vs Gram negative:
GRAM POSITIVE: (1) Those bacteria which are not decolorized are stained PURPLE (2) have THICK walls which are composed of proteins and cross-linked mucopeptides. GRAM NEGATIVE: (1) The decolorized bacteria that take up safranin are stained RED. (2) cell walls are complex multilayered structured that contain lipids in addition to proteins and mucopeptides. |
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What is Gram-Positive and describe its appearance.
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GRAM POSITIVE:
(1) Those bacteria which are not decolorized are stained PURPLE (2) have THICK walls which are composed of proteins and cross-linked mucopeptides. |
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What is Gram-Negative and describe its appearance
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GRAM NEGATIVE:
(1) The decolorized bacteria that take up safranin are stained RED. (2) cell walls are complex multilayered structured that contain lipids in addition to proteins and mucopeptides. |
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Is this Gram-Positive or Gram-Negative bacteria?
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Gram Positive
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Is this Gram-Positive or Gram-Negative?
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Gram-Negative
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List 5 Gram-Positive bacteria
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(1) Bacillus thuringiensis
(2) Staphylococcus aureus (3) Streptococcus agalacitiae (4) Streptococcus lactis (5) Streptococcus pneumoniae |
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List 5 Gram-Negative bacteria
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(1) Enterobacter aerogenes
(2) Escherichia Coli (3) Klebsiella pneumoniae (4) Proteus Vulgaris (5) Rhodospirillum Rubum (5) |